ArticleDevelopmental biology2021
Conditional gene knockdowns in sea urchins using caged morpholinos.
Article in Developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Wnt dynamics at the blastopore and stomodeum during sea urchin gastrulation.Development (Cambridge, England) · 2026Article
- An RNA interference approach for functional studies in the sea urchin and its use in analysis of nodal signaling gradients.Developmental biology · 2024Article
- An RNA interference approach for functional studies in the sea urchin and its use in analysis of Nodal signaling gradients.bioRxiv : the preprint server for biology · 2024Article
- Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function.Angewandte Chemie (International ed. in English) · 2024Article
- Genome-wide identification and spatiotemporal expression analysis of cadherin superfamily members in echinoderms.EvoDevo · 2023Article
- Antisense Oligonucleotide Activation via Enzymatic Antibiotic Resistance Mechanism.ACS chemical biology · 2023Article
- Light-Activated Translation of Different mRNAs in Cells via Wavelength-Dependent Photouncaging.Angewandte Chemie (International ed. in English) · 2023Article
- Enzymatic Modification of the 5' Cap with Photocleavable ONB-Derivatives Using GlaTgs V34A.Chembiochem : a European journal of chemical biology · 2023Article
- An optimized Tet-On system for conditional control of gene expression in sea urchins.Development (Cambridge, England) · 2023Article
- Bicyclic Caged Morpholino Oligonucleotides for Optical Gene Silencing.Chembiochem : a European journal of chemical biology · 2022Article
- Optical Control of MicroRNA Function in Zebrafish Embryos.Journal of the American Chemical Society · 2022Article
- Photocaged 5' cap analogues for optical control of mRNA translation in cells.Nature chemistry · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Small Molecule Control of Morpholino Antisense Oligonucleotide Function through Staudinger Reduction.Journal of the American Chemical Society · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Echinoderms are important experimental models for analyzing embryonic development, but a lack of spatial and temporal control over gene perturbations has hindered developmental studies using these animals. Morpholino antisense oligonucleotides (MOs) have been used successfully by the echinoderm research community for almost two decades, and MOs remain the most widely used tool for acute gene knockdowns in these organisms. Echinoderm embryos develop externally and are optically transparent, making them ideally-suited to many light-based approaches for analyzing and manipulating development. Studies using zebrafish embryos have demonstrated the effectiveness of photoactivatable (caged) MOs for conditional gene knockdowns. Here we show that caged MOs, synthesized using nucleobase-caged monomers, provide light-regulated control over gene expression in sea urchin embryos. Our work provides the first robust approach for conditional gene silencing in this prominent model system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.