Evidence map›Paper›PMID 33683893›Full record

ArticleThe journal of physical chemistry. B2021

Quantum Dots for Improved Single-Molecule Localization Microscopy.

Jennifer M Urban, Wesley Chiang, Jennetta W Hammond, Nicole M B Cogan, Angela Litzburg, Rebeckah Burke, Harry A Stern, Harris A Gelbard, Bradley L Nilsson, Todd D Krauss

Open access · greenAbstract read
In one paragraph

Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jennifer M UrbanDepartment of Chemistry, University of Rochester, Rochester, New York 14627-0216, United States.ORCID 0000-0002-8987-5991
Wesley ChiangDepartment of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, United states.
Jennetta W HammondCenter for Neurotherapeutics Discovery and Department of Neurology, University of Rochester Medical Center, Rochester, New York 14642, United states.
Nicole M B CoganDepartment of Chemistry, University of Rochester, Rochester, New York 14627-0216, United States.
Angela LitzburgCenter for Neurotherapeutics Discovery and Department of Neurology, University of Rochester Medical Center, Rochester, New York 14642, United states.
Rebeckah BurkeDepartment of Chemistry, University of Rochester, Rochester, New York 14627-0216, United States.
Harry A SternCenter for Integrated Research and Computing, University of Rochester, Rochester, New York 14627-0216, United States.
Harris A GelbardCenter for Neurotherapeutics Discovery and Department of Neurology, University of Rochester Medical Center, Rochester, New York 14642, United states.
Bradley L NilssonDepartment of Chemistry, University of Rochester, Rochester, New York 14627-0216, United States.ORCID 0000-0003-1193-3693
Todd D KraussDepartment of Chemistry, University of Rochester, Rochester, New York 14627-0216, United States.ORCID 0000-0002-4860-874X
University of Rochester · USUniversity of Rochester Medical Center · US

Funding

Virology/Immunology CoreP30AI078498 · NIAID · UNIVERSITY OF ROCHESTER · PI DEWHURST, STEPHEN · 2008 to 2018
$14.6M
Immunovascular interactions in postoperative delirium superimposed on dementia (DSD).RF1AG079138 · NIA · DUKE UNIVERSITY · PI GELBARD, HARRIS A, TERRANDO, NICCOLO · 2022 to 2025
$3.6M
Novel Kinase and Nanoformulated Protease Inhibitors for Eradication of CNS HIV-1R01MH104147 · NIMH · UNIVERSITY OF ROCHESTER · PI GELBARD, HARRIS A · 2014 to 2018
$3.2M
Novel Peptide/siRNA Nanoparticles for Treatment of Acute Lung InjuryR01HL138538 · NHLBI · UNIVERSITY OF ROCHESTER · PI DEAN, DAVID A, NILSSON, BRADLEY L. · 2017 to 2020
$2.2M
NHLBI NIH HHS R01 HL138538NIAID NIH HHS P30 AI078498NIA NIH HHS RF1 AG079138NIMH NIH HHS R01 MH104147
6 · The paper itself

Abstract

Colloidal semiconductor quantum dots (QDs) have long established their versatility and utility for the visualization of biological interactions. On the single-particle level, QDs have demonstrated superior photophysical properties compared to organic dye molecules or fluorescent proteins, but it remains an open question as to which of these fundamental characteristics are most significant with respect to the performance of QDs for imaging beyond the diffraction limit. Here, we demonstrate significant enhancement in achievable localization precision in QD-labeled neurons compared to neurons labeled with an organic fluorophore. Additionally, we identify key photophysical parameters of QDs responsible for this enhancement and compare these parameters to reported values for commonly used fluorophores for super-resolution imaging.

Indexed as

Quantum DotsFluorescent DyesMicroscopy, FluorescenceSemiconductorsSingle Molecule ImagingFluorescent Dyes

Identifiers

PMID33683893
PMCPMC8080873
OpenAlexW3133831153

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.