ArticleeLife2021
Relationship between simultaneously recorded spiking activity and fluorescence signal in GCaMP6 transgenic mice.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
118 citing papers in PubMed.
- Sensory plasticity of dorsal horn silent neurons as a critical mechanism for neuropathic pain.Nature communications · 2026Article
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- A versatile platform for two-photon neuronal population voltage imaging across cortical depths.Nature methods · 2026Article
- Temporal drift of sleep-wake representations in hypothalamic neuronal ensembles.Nature communications · 2026Article
- Spike inference from calcium imaging data acquired with GCaMP8 indicators.Nature methods · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Large-scale electrophysiology at single-spike resolution.Nature reviews. Neuroscience · 2026Review
- Simultaneous Whole-Cell Recording and Calcium Imaging Does Not Reveal Electrically Coupled Neurons ineNeuro · 2026Article
- Nonuniform spike count shared variability in the mouse early visual system.Journal of neurophysiology · 2026Article
- Astrocyte Proximity Protects Synapses From Human Amyloid-Beta Induced Degeneration in a Mouse Ex Vivo Model of Early Alzheimer's Disease.The European journal of neuroscience · 2026Article
- NPAS4 refines spatial and temporal firing in CA1 pyramidal neurons.bioRxiv : the preprint server for biology · 2026Article
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Simultaneous recording of spikes and calcium signals in odor-evoked responses ofJournal of neurogenetics · 2025Article
- Chronic alteration of CabioRxiv : the preprint server for biology · 2025Article
- Improved inference of latent neural states from calcium imaging data.bioRxiv : the preprint server for biology · 2025Article
- Temporal coding carries more stable cortical visual representations than firing rate over time.Nature communications · 2025Article
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Article
- Unraveling the neural code: analysis of large-scale two-photon microscopy data.Microscopy (Oxford, England) · 2025Review
- Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Fluorescent calcium indicators are often used to investigate neural dynamics, but the relationship between fluorescence and action potentials (APs) remains unclear. Most APs can be detected when the soma almost fills the microscope's field of view, but calcium indicators are used to image populations of neurons, necessitating a large field of view, generating fewer photons per neuron, and compromising AP detection. Here, we characterized the AP-fluorescence transfer function in vivo for 48 layer 2/3 pyramidal neurons in primary visual cortex, with simultaneous calcium imaging and cell-attached recordings from transgenic mice expressing GCaMP6s or GCaMP6f. While most APs were detected under optimal conditions, under conditions typical of population imaging studies, only a minority of 1 AP and 2 AP events were detected (often <10% and ~20-30%, respectively), emphasizing the limits of AP detection under more realistic imaging conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.