ArticleFrontiers in bioengineering and biotechnology2021
Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films.
Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- Temporal Interference Stimulation Enhances Neural Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- PEDOT:PSS-A Key Material for Bioelectronics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- PEDOT:PSS in peripheral nerve injury repair: electrical stimulation, neural interfaces, and neural regenerative mechanisms.Materials today. Bio · 2026Review
- Biobased Polymers in Printed Electronics: From Renewable Resources to Functional Devices.Polymers · 2026Review
- The Bionic Interface: Considering the Material Mediated Electrical Stimulation of Stem Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Combined effects of red light and direct-current electric fields on neurite growth in 3D neural cell cultures.Scientific reports · 2025Article
- Electrical Stimulation of Cells: Drivers, Technology, and Effects.Chemical reviews · 2025Review
- Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes.Regenerative medicine · 2025Article
- Inkjet-printed transparent electrodes: Design, characterization, and initial in vivo evaluation for brain stimulation.PloS one · 2025Article
- Transcriptomic Analysis Reveals the Heterogeneous Role of Conducting Films Upon Electrical Stimulation.Advanced healthcare materials · 2024Article
- Understanding Thermodynamics and Kinetics of PEDOT:PSS Using ATR-FTIR and Density Functional Theory.ACS omega · 2024Article
- Bioelectronic Neural Interfaces: Improving Neuromodulation Through Organic Conductive Coatings.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- sFlt-1 impairs neurite growth and neuronal differentiation in SH-SY5Y cells and human neurons.Bioscience reports · 2024Article
- Development of a Cell Culture Chamber for Investigating the Therapeutic Effects of Electrical Stimulation on Neural Growth.Biomedicines · 2024Article
- Leveraging Biomaterial Platforms to Study Aging-Related Neural and Muscular Degeneration.Biomolecules · 2024Review
- Resorbable conductive materials for optimally interfacing medical devices with the living.Frontiers in bioengineering and biotechnology · 2024Review
- Synergy of Nanotopography and Electrical Conductivity of PEDOT/PSS for Enhanced Neuronal Development.ACS applied materials & interfaces · 2023Article
- Biophysical control of plasticity and patterning in regeneration and cancer.Cellular and molecular life sciences : CMLS · 2023Review
- Biohybrid neural interfaces: improving the biological integration of neural implants.Chemical communications (Cambridge, England) · 2023Review
- PEDOT:PSS-coated platinum electrodes for neural stimulation.APL bioengineering · 2023Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. Recent studies have shown that electrical stimulation improves neuronal differentiation of stem cells populations, highlighting the importance of the development of electroconductive biocompatible materials for NSC culture and differentiation for tissue engineering and regenerative medicine. Here, we report the use of the conjugated polymer poly(3,4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS CLEVIOS P AI 4083) for the manufacture of conductive substrates. Two different protocols, using different cross-linkers (3-glycidyloxypropyl)trimethoxysilane (GOPS) and divinyl sulfone (DVS) were tested to enhance their stability in aqueous environments. Both cross-linking treatments influence PEDOT:PSS properties, namely conductivity and contact angle. However, only GOPS-cross-linked films demonstrated to maintain conductivity and thickness during their incubation in water for 15 days. GOPS-cross-linked films were used to culture ReNcell-VM under different electrical stimulation conditions (AC, DC, and pulsed DC electrical fields). The polymeric substrate exhibits adequate physicochemical properties to promote cell adhesion and growth, as assessed by Alamar Blue® assay, both with and without the application of electric fields. NSCs differentiation was studied by immunofluorescence and quantitative real-time polymerase chain reaction. This study demonstrates that the pulsed DC stimulation (1 V/cm for 12 days), is the most efficient at enhancing the differentiation of NSCs into neurons.
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