ArticleBiomedical optics express2021
Three-dimensional characterization of collagen remodeling in cell-seeded collagen scaffolds via polarization second harmonic generation.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nonlinear Microscopy of ECM Remodeling in Renal and Vascular Tissues: A Systematic Review Integrating Human AVF Imaging.Medicina (Kaunas, Lithuania) · 2026Pooled it
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Assessment of aqueous graphene as a cancer therapeutics delivery system.Scientific reports · 2025Article
- Miniaturized Iontronic Micropipettes for Precise and Dynamic Ionic Modulation of Neuronal and Astrocytic Activity.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging.Scientific reports · 2025Article
- Flow-Induced Shear Stress Combined with Microtopography Inhibits the Differentiation of Neuro-2a Cells.Micromachines · 2025Article
- Profiling of collagen and extracellular matrix deposition from cell culture using in vitro ExtraCellular matrix mass spectrometry imaging (ivECM-MSI).Matrix biology plus · 2024Article
- Water-Soluble Bimodal Magnetic-Fluorescent Radical Dendrimers as Potential MRI-FI Imaging Probes.ACS applied materials & interfaces · 2024Article
- Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion.Biomedical optics express · 2024Article
- Label-free optical imaging of cell function and collagen structure for cell-based therapies.Current opinion in biomedical engineering · 2023Article
- Assessment of Collagen in Translational Models of Lung Research.Advances in experimental medicine and biology · 2023Article
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Authors and funding
9 authors.
Funding
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Abstract
In this study, we use non-linear imaging microscopy to characterize the structural properties of porous collagen-GAG scaffolds (CGS) seeded with human umbilical vein endothelial cells (HUVECs), as well as human mesenchymal stem cells (hMSCs), a co-culture previously reported to form vessel-like structures inside CGS. The evolution of the resulting tissue construct was monitored over 10 days via simultaneous two- and three-photon excited fluorescence microscopy. Time-lapsed 2- and 3-photon excited fluorescence imaging was utilized to monitor the temporal evolution of the vascular-like structures up to 100 µm inside the scaffold up to 10 days post-seeding. 3D polarization-dependent second harmonic generation (PSHG) was utilized to monitor collagen-based scaffold remodeling and determine collagen fibril orientation up to 200 µm inside the scaffold. We demonstrate that polarization-dependent second harmonic generation can provide a novel way to quantify the reorganization of the collagen architecture in CGS simultaneously with key biomechanical interactions between seeded cells and CGS that regulate the formation of vessel-like structures inside 3D tissue constructs. A comparison between samples at different days in vitro revealed that gradually, the scaffolds developed an orthogonal net-like architecture, previously found in real skin.
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Registered trials
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