Evidence map›Paper›PMID 33679256›Full record

ArticleIEEE sensors journal2021

The C-terminus of the mu opioid receptor is critical in G-protein interaction as demonstrated by a novel graphene biosensor.

Chengyu Wen, Bernard Selling, Alexei Yeliseev, Jin Xi, Jose Manuel Perez-Aguilar, Zhaoli Gao, Jeffery G Saven, A T Charlie Johnson, Renyu Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in IEEE sensors journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Activation dynamics of a water-soluble human mu-opioid receptor.The Journal of biological chemistry · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chengyu WenDepartment of Electrical and Systems Engineering, University of Pennsylvania, Philadelphia, PA 19104 USA.
Bernard SellingDepartment of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia PA 19104, USA.
Alexei YeliseevNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20852, USA.
Jin XiDepartment of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia PA 19104, USA.
Jose Manuel Perez-AguilarDepartment of Chemistry, University of Pennsylvania, Philadelphia PA 19104, USA.
Zhaoli GaoDepartment of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong.
Jeffery G SavenDepartment of Chemistry, University of Pennsylvania, Philadelphia PA 19104, USA.
A T Charlie JohnsonDepartment of Physics and Astronomy, University of Pennsylvania, Philadelphia PA 19104, USA.
Renyu LiuDepartment of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia PA 19104, USA.

Funding

Water soluble variants of the human mu opioid receptorR01GM111421 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI LIU, RENYU · 2014 to 2018
$1.7M
NIGMS NIH HHS R01 GM111421
6 · The paper itself

Abstract

Several water-soluble variants of the human mu opioid receptor (wsMORs) have been designed and expressed, which enables the detection of opioids in the nM to pM range using biosensing platforms. The tools previously developed allowed us to investigate MOR and G-protein interactions in a lipid free system to demonstrate that the lipid bilayer might not be essential for the G-protein recognition and binding. In this study, we are able to investigate G-protein interactions with MOR by using graphene enabled technology, in a lipid free system, with a high sensitivity in a real time manner. A new wsMOR with the native C-terminus was designed, expressed and then immobilized on the surfaces of scalable graphene field effect transistor (GFET)-based biosensors, enabling the recording of wsMOR/G-protein interaction with an electronic readout. G-protein only interacts with the wsMOR in the presence of the native MOR C-terminus with a K

Indexed as

BiosensorsC-terminusG-proteingraphenemu opioid receptorwater-soluble

Identifiers

PMID33679256
PMCPMC7935050

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.