Evidence map›Paper›PMID 33675405›Full record

ArticleCurrent microbiology2021

Clostridium butyricum Supernatant Regulates the Expression of RORγt in HCT-116 Cells by Inhibiting the TLR2/MyD88/NF-κB Signaling Pathway.

Ying Xie, Linyan Zhou, Hui Li, Yan Li

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. The effects of different doses ofVeterinary and animal science · 2026
    Article
  2. Probiotic Roles ofAnimals : an open access journal from MDPI · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ying XieDepartment of Gastroenterology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, 110004, Liaoning, China.ORCID http://orcid.org/0000-0001-8226-4242
Linyan ZhouDepartment of Gastroenterology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, 110004, Liaoning, China.ORCID http://orcid.org/0000-0002-9403-2809
Hui LiDepartment of Gastroenterology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, 110004, Liaoning, China.ORCID http://orcid.org/0000-0002-7521-7648
Yan LiDepartment of Gastroenterology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Shenyang, 110004, Liaoning, China. liy@sj-hospital.org.ORCID http://orcid.org/0000-0002-0550-3155
China Medical University · CN

Funding

Shenyang Science and Technology Bureau F13-316-1-24
6 · The paper itself

Abstract

In this study, we treated HCT-116 cells with Clostridium butyricum (C. butyricum) supernatant and observed its effects on the TLR2/MyD88/NF-κB signaling pathway and RORγt, to further explore the possible immune regulatory mechanism of C. butyricum. Our results showed that C. butyricum supernatant downregulated the mRNA and protein levels of TLR2, MyD88, NF-κBp65, and RORγt in HCT-116 cells and the protein levels of phospho-NF-κBp65. Partial blockage of TLR2 by CD282 weakened the inhibitory effects of C. butyricum supernatant on the above pathway components. Those component levels were still inhibited by C. butyricum supernatant after Pam3CSK4 activation of TLR2. In summary, C. butyricum supernatant can inhibit the TLR2/MyD88/NF-κB signaling pathway and the expression of RORγt in HCT-116 cells. These effects are at least partly achieved through inhibition of TLR2.

Indexed as

Clostridium butyricumNF-kappa BHCT116 CellsHumansMyeloid Differentiation Factor 88Nuclear Receptor Subfamily 1, Group F, Member 3Signal TransductionToll-Like Receptor 2Myeloid Differentiation Factor 88NF-kappa BNuclear Receptor Subfamily 1, Group F, Member 3TLR2 protein, humanToll-Like Receptor 2

Identifiers

PMID33675405
OpenAlexW3135899454

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.