ArticleNature communications2021
Inherently confinable split-drive systems in Drosophila.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 93 citations in OpenAlex.
- Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load inbioRxiv : the preprint server for biology · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Engineering resilient gene drives for sustainable malaria control by predicting, testing and overcoming target site resistance in Anopheles gambiae.PLoS biology · 2026Article
- Self-limiting population suppression gene drive design in the West Nile vector mosquito, Culex quinquefasciatus.Nature communications · 2026Article
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- A temperature-sensitive CRISPR-Cas12a system for sterile insect technique.Nature communications · 2025Article
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- Article
- Synthetic homing endonuclease gene drives to revolutionise Aedes aegypti biocontrol - game changer or pipe dream?Current opinion in insect science · 2025Review
- Gene drives: an alternative approach to malaria control?Gene therapy · 2025Review
- A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population.Nature communications · 2024Article
- Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila.Nature communications · 2024Article
- Expanding the CRISPR base editing toolbox in Drosophila melanogaster.Communications biology · 2024Article
- Advancements and Future Prospects of CRISPR-Cas-Based Population Replacement Strategies in Insect Pest Management.Insects · 2024Review
- Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails.Nature communications · 2024Article
- Modelling daisy quorum drive: A short-term bridge across engineered fitness valleys.PLoS genetics · 2024Article
- A small-molecule approach to restore female sterility phenotype targeted by a homing suppression gene drive in the fruit pest Drosophila suzukii.PLoS genetics · 2024Article
- Developmental progression of DNA double-strand break repair deciphered by a single-allele resolution mutation classifier.Nature communications · 2024Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
CRISPR-based gene-drive systems, which copy themselves via gene conversion mediated by the homology-directed repair (HDR) pathway, have the potential to revolutionize vector control. However, mutant alleles generated by the competing non-homologous end-joining (NHEJ) pathway, resistant to Cas9 cleavage, can interrupt the spread of gene-drive elements. We hypothesized that drives targeting genes essential for viability or reproduction also carrying recoded sequences that restore endogenous gene functionality should benefit from dominantly-acting maternal clearance of NHEJ alleles combined with recessive Mendelian culling processes. Here, we test split gene-drive (sGD) systems in Drosophila melanogaster that are inserted into essential genes required for viability (rab5, rab11, prosalpha2) or fertility (spo11). In single generation crosses, sGDs copy with variable efficiencies and display sex-biased transmission. In multigenerational cage trials, sGDs follow distinct drive trajectories reflecting their differential tendencies to induce target chromosome damage and/or lethal/sterile mosaic Cas9-dependent phenotypes, leading to inherently confinable drive outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.