ArticleNature communications2021
Single-cell measurement of plasmid copy number and promoter activity.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed.
- Stabilization of microbial cell population for long-term plasmid production in continuous biomanufacturing.Bioprocess and biosystems engineering · 2026Article
- Genetic Toolbox Expansion Enables Constitutively Fluorescent Lacticaseibacillus rhamnosus for Functional Microbiome Research.Microbial biotechnology · 2026Article
- Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for coronavirus.Poultry science · 2026Article
- PlasmidGPT: A generative framework for plasmid analysis and generation.Science advances · 2026Article
- Bacterial Schlafen proteins mediate phage defence.Nature microbiology · 2026Article
- High-throughput methods enabling random duplications, deletions, or nucleotide-constrained mutagenesis of entire DNA motifs.Nucleic acids research · 2026Article
- Revelation of core promoter elements and bidirectional regulation of Geminivirus genes in Escherichia coli.Virus research · 2026Article
- SHARK: A Specialized Host for Assembling R6K Plasmids.ACS synthetic biology · 2026Article
- A conjugal gene drive-like system efficiently suppresses antibiotic resistance in a bacterial population.npj antimicrobials and resistance · 2026Article
- Engineering plasmids with synthetic origins of replication.Nature communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- Dynamical memory underlies prolonged plasmid persistence after transient antibiotic treatment.bioRxiv : the preprint server for biology · 2025Article
- Effectiveness of CRISPR-Cas in sensitizing bacterial populations with plasmid-encoded antimicrobial resistance.Genetics · 2025Article
- Escherichia coli strain O55 contains two cryptic plasmids that depend on each other to replicate.Archives of microbiology · 2025Article
- Congruence between noise and plasticity in protein expression.Scientific reports · 2025Article
- Preventing Multimer Formation in Commonly Used Synthetic Biology Plasmids.ACS synthetic biology · 2025Article
- Deletion of pcnB affects antibiotic susceptibility in resistant Escherichia coli by reducing copy number of ColE1-family plasmids.Scientific reports · 2025Article
- Construction and Characterization of MoClo-Compatible Vectors for Modular Protein Expression inACS synthetic biology · 2025Article
- Partitioning of a 2-bit hash function across 66 communicating cells.Nature chemical biology · 2025Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
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