ArticleNature communications2021
De novo design of a reversible phosphorylation-dependent switch for membrane targeting.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Enzyme-Free Phosphorylation with Kinetic Gating in a De Novo Coiled-Coil System.Journal of the American Chemical Society · 2026Article
- Biomimetic peptide self-assembly: interfacing with biomacromolecules to regulate cellular signaling.Experimental & molecular medicine · 2026Review
- Sequence Prediction, Structural Modelling and the Challenges of Plasticity in Coiled Coils.Sub-cellular biochemistry · 2026Review
- Chemically active wetting.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Selectivity of membrane-active peptides: the role of electrostatics and other membrane biophysical properties.Biophysical reviews · 2025Review
- The impact of IDR phosphorylation on the RNA binding profiles of proteins.Trends in genetics : TIG · 2024Review
- A de novo designed coiled coil-based switch regulates the microtubule motor kinesin-1.Nature chemical biology · 2024Article
- Quantitative phosphoproteomics reveals molecular pathway network alterations in human early-stage primary hepatic carcinomas: potential for 3P medical approach.The EPMA journal · 2023Article
- Lipophilic Anchors that Embed Bioconjugates in Bilayer Membranes: A Review.Bioconjugate chemistry · 2023Review
- Design and Selection of Heterodimerizing Helical Hairpins for Synthetic Biology.ACS synthetic biology · 2023Article
- Rational Design of Phosphorylation-Responsive Coiled Coil-Peptide Assemblies.ACS synthetic biology · 2023Article
- Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression.ACS synthetic biology · 2023Article
- How phosphorylation impacts intrinsically disordered proteins and their function.Essays in biochemistry · 2022Article
- A glutamine-based single α-helix scaffold to target globular proteins.Nature communications · 2022Article
- Redox- and metal-directed structural diversification in designed metalloprotein assemblies.Chemical communications (Cambridge, England) · 2022Article
- α-Helix stabilization by co-operative side chain charge-reinforced interactions to phosphoserine in a basic kinase-substrate motif.The Biochemical journal · 2022Article
- Pharmacological Functionalization of Protein-Based Nanorobots as a Novel Tool for Drug Delivery in Cancer.ACS pharmacology & translational science · 2021Article
- DE-STRESS: a user-friendly web application for the evaluation of protein designs.Protein engineering, design & selection : PEDS · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Modules that switch protein-protein interactions on and off are essential to develop synthetic biology; for example, to construct orthogonal signaling pathways, to control artificial protein structures dynamically, and for protein localization in cells or protocells. In nature, the E. coli MinCDE system couples nucleotide-dependent switching of MinD dimerization to membrane targeting to trigger spatiotemporal pattern formation. Here we present a de novo peptide-based molecular switch that toggles reversibly between monomer and dimer in response to phosphorylation and dephosphorylation. In combination with other modules, we construct fusion proteins that couple switching to lipid-membrane targeting by: (i) tethering a 'cargo' molecule reversibly to a permanent membrane 'anchor'; and (ii) creating a 'membrane-avidity switch' that mimics the MinD system but operates by reversible phosphorylation. These minimal, de novo molecular switches have potential applications for introducing dynamic processes into designed and engineered proteins to augment functions in living cells and add functionality to protocells.
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Registered trials
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