Evidence map›Paper›PMID 33673130›Full record

ArticleBiomolecules2021

Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer.

Markus Kilisch, Hansjörg Götzke, Maja Gere-Becker, Alexander Crauel, Felipe Opazo, Steffen Frey

Open access · goldAbstract read
In one paragraph

Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. State-of-the-Art and Future Directions in Structural Proteomics.Molecular & cellular proteomics : MCP · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Markus KilischNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.
Hansjörg GötzkeNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.ORCID 0000-0002-2237-4260
Maja Gere-BeckerNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.
Alexander CrauelNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.
Felipe OpazoNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.ORCID 0000-0002-4968-9713
Steffen FreyNanoTag Biotechnologies GmbH, Rudolf-Wissell-Straße 28a, 37079 Göttingen, Germany.ORCID 0000-0002-5134-9961
Universitätsmedizin Göttingen · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epitope tags are widely employed as tools to detect, purify and manipulate proteins in various experimental systems. We recently introduced the ALFA-tag together with two ALFA-specific single-domain antibodies (sdAbs), NbALFA and NbALFA

Indexed as

Cold TemperatureBuffersChromatography, AffinityEpitopesImmunoprecipitationProteinsSingle-Domain AntibodiesBuffersEpitopesProteinsSingle-Domain AntibodiesaffinityALFA SelectorALFA systemcold-elutableepitope tagimmunoprecipitationnanobodynative elutionsdAbssynthetic library

Identifiers

PMID33673130
PMCPMC7918568
OpenAlexW3129320664

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.