ReviewMicroorganisms2021
DNA Metabarcoding for the Characterization of Terrestrial Microbiota-Pitfalls and Solutions.
Review in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Lignin enrichment selects specialized fungal communities from contrasting soils.Sustainable microbiology · 2026Article
- Metabarcoding Reveals Fungal Diversity in the Root and Rhizosphere ofInternational journal of microbiology · 2026Article
- The Desert in Bottles: The Culturable Microbiome of the Atacama Desert's Grit Crust.Environmental microbiology reports · 2025Article
- A novel two-step metabarcoding approach improves soil microbiome biodiversity assessment.Scientific reports · 2025Article
- Article
- Consequences of Domestication on Gut Microbiome: A Comparative Analysis Between Wild Boars and Domestic Pigs.Animals : an open access journal from MDPI · 2025Article
- Role of Plants and Urban Soils in Carbon Stock: Status, Modulators, and Sustainable Management Practices.Plants (Basel, Switzerland) · 2025Review
- Next-generation sequencing applications in food science: fundamentals and recent advances.Frontiers in bioengineering and biotechnology · 2025Review
- Moderate thinning enhances soil carbon-nitrogen cycling and microbial diversity in degraded mixed forests.Frontiers in microbiology · 2025Article
- Potential Plant-To-Plant Transmission: Shared Endophytic Bacterial Community Between Ziziphus lotus and Its Parasite Cuscuta epithymum.Microbial ecology · 2024Article
- Seasonal soil health dynamics in soy-wheat relay intercropping.Scientific reports · 2024Article
- Stratified Effects of Tillage and Crop Rotations on Soil Microbes in Carbon and Nitrogen Cycles at Different Soil Depths in Long-Term Corn, Soybean, and Wheat Cultivation.Microorganisms · 2024Article
- Article
- Identification of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections.Parasites & vectors · 2023Article
- Phylogenetic diversity and functional potential of the microbial communities along the Bay of Bengal coast.Scientific reports · 2023Article
- Unlocking secrets of microbial ecotoxicology: recent achievements and future challenges.FEMS microbiology ecology · 2023Review
- Using nanopore sequencing to identify fungi from clinical samples with high phylogenetic resolution.Scientific reports · 2023Article
- Culture-Dependent and Metabarcoding Characterization of the Sugar Beet (Microorganisms · 2023Article
- AirDNA sampler: An efficient and simple device enabling high-yield, high-quality airborne environment DNA for metagenomic applications.PloS one · 2023Article
- New Insights into the Effect of Fipronil on the Soil Bacterial Community.Microorganisms · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soil-borne microbes are major ecological players in terrestrial environments since they cycle organic matter, channel nutrients across trophic levels and influence plant growth and health. Therefore, the identification, taxonomic characterization and determination of the ecological role of members of soil microbial communities have become major topics of interest. The development and continuous improvement of high-throughput sequencing platforms have further stimulated the study of complex microbiota in soils and plants. The most frequently used approach to study microbiota composition, diversity and dynamics is polymerase chain reaction (PCR), amplifying specific taxonomically informative gene markers with the subsequent sequencing of the amplicons. This methodological approach is called DNA metabarcoding. Over the last decade, DNA metabarcoding has rapidly emerged as a powerful and cost-effective method for the description of microbiota in environmental samples. However, this approach involves several processing steps, each of which might introduce significant biases that can considerably compromise the reliability of the metabarcoding output. The aim of this review is to provide state-of-the-art background knowledge needed to make appropriate decisions at each step of a DNA metabarcoding workflow, highlighting crucial steps that, if considered, ensures an accurate and standardized characterization of microbiota in environmental studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.