ArticleViruses2021
Standard Bacteriophage Purification Procedures Cause Loss in Numbers and Activity.
Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Isolation and characterization of bacteriophages against antibiotic-resistanceIranian journal of microbiology · 2026Article
- A New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration and Partial Purification of Recombinant Prepromelittin Produced by Komagataella phaffii.Biotechnology and applied biochemistry · 2026Article
- High throughput chromatographic ultra-purification of virus-like particles for downstream viromics.bioRxiv : the preprint server for biology · 2026Article
- Systematic scale-up and enhanced purification of marine cyanophage P-SSP7.Frontiers in microbiology · 2026Article
- Article
- Phage Therapy: Application and Related Problems-A Review.Life (Basel, Switzerland) · 2025Review
- Prophage border curation reveals horizontal transfer of lysogeny-related elements between filamentous and double jelly-roll phages.Communications biology · 2025Article
- The N-Terminal Domain of Tailspike Depolymerases Affects the Replication Efficiency of SyntheticInternational journal of molecular sciences · 2025Article
- Beware of host immune responses towards bacteriophages potentially impacting phage therapy.Veterinary research · 2025Article
- Replacement of the Genomic Scaffold Improves the Replication Efficiency of SyntheticInternational journal of molecular sciences · 2025Article
- Mystique, a broad host range Acinetobacter phage, reveals the impact of culturing conditions on phage isolation and infectivity.PLoS pathogens · 2025Article
- Transcytosis of T4 Bacteriophage Through Intestinal Cells Enhances Its Immune Activation.Viruses · 2025Article
- A Lambda-evo (λApplied microbiology and biotechnology · 2025Article
- Article
- SXP01: a novel bacteriophage for combatingFrontiers in microbiology · 2025Article
- Genomic transfer via membrane vesicle: a strategy of giant phage phiKZ for early infection.Journal of virology · 2024Article
- Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform.Scientific reports · 2024Article
- Detection, isolation and characterization of phage-host complexes using BONCAT and click chemistry.Frontiers in microbiology · 2024Article
- Free DNA partially clarifies discrepancies between qPCR and the conventional phage quantification method.PloS one · 2024Article
- Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy.Methods in molecular biology (Clifton, N.J.) · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For decades, bacteriophage purification has followed structured protocols focused on generating high concentrations of phage in manageable volumes. As research moves toward understanding complex phage populations, purification needs have shifted to maximize the amount of phage while maintaining diversity and activity. The effects of standard phage purification procedures such as polyethylene glycol (PEG) precipitation and cesium chloride (CsCl) density gradients on both diversity and activity of a phage population are not known. We have examined the effects of PEG precipitation and CsCl density gradients on a number of known phage (M13, T4, and ΦX 174) of varying structure and size, individually and as mixed sample. Measurement of phage numbers and activity throughout the purification process was performed. We demonstrate that these methods, used routinely to generate "pure" phage samples, are in fact detrimental to retention of phage number and activity; even more so in mixed phage samples. As such, minimal amounts of processing are recommended to introduce less bias and maintain more of a phage population.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.