ArticleAntibiotics (Basel, Switzerland)2021
Development of Europium-Sensitized Fluorescence-Based Method for Sensitive Detection of Oxytetracycline in Citrus Tissues.
Article in Antibiotics (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Excitation-depended fluorescence emission of boron-doped graphene quantum dot as an optical probe for detection of oxytetracycline in food and information encryption patterns.Mikrochimica acta · 2023Article
- Detection of Oxytetracycline in Citrus Phloem and Xylem Saps Using Europium-Based Method.Antibiotics (Basel, Switzerland) · 2021Article
- Citrate Mediated Europium-Based Detection of Oxytetracycline in Citrus Tissues.Antibiotics (Basel, Switzerland) · 2021Article
- Controlling Citrus Huanglongbing: Green Sustainable Development Route Is the Future.Frontiers in plant science · 2021Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Antimicrobial compounds have been successfully used to control many plant and animal diseases. Recently, oxytetracycline (OTC) and streptomycin have been approved for the treatment of Huanglongbing in citrus. Since the application of OTC is under strict regulations, several methods have been developed to determine and monitor its levels in the environment including high-performance liquid chromatography, ELISA, colorimetric, and fluorometric assays. In this study, we developed a fluorometric method for the determination of OTC in plant tissues based on its complexation with europium. Our preliminary trials showed that phenols and flavonoids interfere with the europium assay by reacting with the sensitizing reagent, cetyltrimethylammonium chloride. Consequently, we used the 60 mg hydrophilic-lipophilic balanced (HLB) cartridges to purify the OTC from the plant matrix. The recovery of OTC from spiked leaf samples was 75 ± 7.6%. Using the 500 mg HLB, we were able to detect 0.3 ppm OTC in the final sample extract, which corresponds to 3 µg g
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Registered trials
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