ArticleMethods and protocols2021
Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR.
Article in Methods and protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Accounting for methodological variabilities in disease outbreak surveillance using wastewater-based epidemiology: a case study with SARS-CoV-2.Analytical and bioanalytical chemistry · 2026Article
- Effect of nucleic acid concentration method on Severe Acute Respiratory Syndrome Coronavirus 2 detection sensitivity in aircraft wastewater.Journal of medical microbiology · 2026Article
- Surveillance of zoonotic respiratory viral infections in animal farms: a model project with a focus on One Health approach-A study protocol.Frontiers in public health · 2026Article
- Trends of adenovirus occurrence in drinking and wastewater treatment plants of the city of Hamadan, Iran.Journal of water and health · 2025Article
- Towards ultra-sensitive and rapid near-source wastewater-based epidemiology.Nature communications · 2025Article
- Exploring the Use of Passive Samplers for the Surveillance of Avian Influenza Viruses in Wetlands: A Laboratory and Field Validation Study.Food and environmental virology · 2025Article
- Temporal dynamics of SARS-CoV-2 detection in wastewater and population infection trends in Mexico City.Frontiers in public health · 2025Article
- Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment.Viruses · 2024Article
- Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity.One health (Amsterdam, Netherlands) · 2024Article
- Article
- Uncovering the presence of CVPD disease in citrus varieties of South Sulawesi, Indonesia: A molecular approach.Journal, genetic engineering & biotechnology · 2024Article
- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Campus source to sink wastewater surveillance of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Current research in microbial sciences · 2024Article
- Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples.FEMS microbes · 2024Article
- Use of wastewater from passenger ships to assess the movement of COVID-19 and other pathogenic viruses across maritime international boundaries.Frontiers in public health · 2024Article
- Evaluation of wastewater surveillance for SARS-CoV-2 in a prison population: a mixed-methods approach.Frontiers in public health · 2024Article
- Article
- Diurnal changes in pathogenic and indicator virus concentrations in wastewater.Environmental science and pollution research international · 2023Article
- VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance.Scientific reports · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Wastewater-based epidemiology has become an important tool for the surveillance of SARS-CoV-2 outbreaks. However, the detection of viruses in sewage is challenging and to date there is no standard method available which has been validated for the sensitive detection of SARS-CoV-2. In this paper, we describe a simple concentration method based on polyethylene glycol (PEG) precipitation, followed by RNA extraction and a one-step quantitative reverse transcription PCR (qRT-PCR) for viral detection in wastewater. PEG-based concentration of viruses is a simple procedure which is not limited by the availability of expensive equipment and has reduced risk of disruption to consumable supply chains. The concentration and RNA extraction steps enable 900-1500× concentration of wastewater samples and sufficiently eliminates the majority of organic matter, which could inhibit the subsequent qRT-PCR assay. Due to the high variation in the physico-chemical properties of wastewater samples, we recommend the use of process control viruses to determine the efficiency of each step. This procedure enables the concentration and the extraction the DNA/RNA of different viruses and hence can be used for the surveillance of different viral targets for the comprehensive assessment of viral diseases in a community.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.