Evidence map›Paper›PMID 33671612›Full record

ArticleInternational journal of molecular sciences2021

Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors.

Kohya Uematsu, Takashi Ushiki, Hajime Ishiguro, Riuko Ohashi, Suguru Tamura, Mari Watanabe, Yoko Fujimoto, Masaki Nagata, Yoichi Ajioka, Tomoyuki Kawase

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kohya UematsuDivision of Dental Implantology, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan.
Takashi UshikiDepartment of Transfusion Medicine, Cell Therapy and Regenerative Medicine, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan.ORCID 0000-0001-5590-2480
Hajime IshiguroDepartment of Hematology, Endocrinology and Metabolism, Faculty of Medicine, Niigata University, Niigata 951-8510, Japan.
Riuko OhashiHistopathology Core Facility, Faculty of Medicine, Niigata University, Niigata 951-8510, Japan.ORCID 0000-0001-5820-7870
Suguru TamuraDepartment of Hematology, Endocrinology and Metabolism, Faculty of Medicine, Niigata University, Niigata 951-8510, Japan.
Mari WatanabeDepartment of Transfusion Medicine, Cell Therapy and Regenerative Medicine, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan.
Yoko FujimotoDepartment of Transfusion Medicine, Cell Therapy and Regenerative Medicine, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan.
Masaki NagataDivision of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan.
Yoichi AjiokaHistopathology Core Facility, Faculty of Medicine, Niigata University, Niigata 951-8510, Japan.
Tomoyuki KawaseDivision of Oral Bioengineering, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan.ORCID 0000-0003-4160-396X

Funding

Japan Society for the Promotion of Science 15H06228, 16K20534 and 19K19048Niigata University Medical and Dental Hospital none
6 · The paper itself

Abstract

Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP

Indexed as

Biocompatible MaterialsAdultAnimalsBone Marrow TransplantationCulture MediaFemaleGreen Fluorescent ProteinsHumansMaleMaterials TestingMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicOsteoclastsOsteogenesisBiocompatible MaterialsCulture MediaGreen Fluorescent Proteinsbone marrow transplantationCD11bgreen fluorescent proteinosteoclastogenesisperiosteal sheetstartrate-resistant acid phosphatase

Identifiers

PMID33671612
PMCPMC7926432

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.