ArticleInternational journal of molecular sciences2021
Hemidesmosome-Related Keratin Filament Bundling and Nucleation.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 40 citations in OpenAlex.
- Dynamics of supracellular keratin bundling and nuclear uncaging in stretched epithelia.Nature physics · 2026Article
- Influence of Machining, Polishing, and Glazing on Surface Properties and Biological Performance of Zirconia and Lithium Disilicate Dental Ceramics.Journal of functional biomaterials · 2025Article
- Hemidesmosomes regulate epidermal differentiation during embryogenesis.bioRxiv : the preprint server for biology · 2025Article
- Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae).Protoplasma · 2025Article
- Mechanics of cell sheets: plectin as an integrator of cytoskeletal networks.Open biology · 2025Review
- Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.The Journal of cell biology · 2023Article
- Recent Advances in Intermediate Filaments-Volume 1.International journal of molecular sciences · 2022Article
- Cortical tension regulates desmosomal morphogenesis.Frontiers in cell and developmental biology · 2022Article
- Loss of α6β4 Integrin-Mediated Hemidesmosomes Promotes Prostate Epithelial Cell Migration by Stimulating Focal Adhesion Dynamics.Frontiers in cell and developmental biology · 2022Article
- Combining Image Restoration and Traction Force Microscopy to Study Extracellular Matrix-Dependent Keratin Filament Network Plasticity.Frontiers in cell and developmental biology · 2022Article
- Intermediate Filaments in Cellular Mechanoresponsiveness: Mediating Cytoskeletal Crosstalk From Membrane to Nucleus and Back.Frontiers in cell and developmental biology · 2022Review
- Increased Expression of Flightless I in Cutaneous Squamous Cell Carcinoma Affects Wnt/β-Catenin Signaling Pathway.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The epithelial cytoskeleton encompasses actin filaments, microtubules, and keratin intermediate filaments. They are interconnected and attached to the extracellular matrix via focal adhesions and hemidesmosomes. To study their interplay, we inhibited actin and tubulin polymerization in the human keratinocyte cell line HaCaT by latrunculin B and nocodazole, respectively. Using immunocytochemistry and time-lapse imaging of living cells, we found that inhibition of actin and tubulin polymerization alone or in combination induced keratin network re-organization albeit differently in each situation. Keratin filament network retraction towards the nucleus and formation of bundled and radial keratin filaments was most pronounced in latrunculin-B treated cells but less in doubly-treated cells and not detectable in the presence of nocodazole alone. Hemidesmosomal keratin filament anchorage was maintained in each instance, whereas focal adhesions were disassembled in the absence of actin filaments. Simultaneous inhibition of actin and tubulin polymerization, therefore, allowed us to dissect hemidesmosome-specific functions for keratin network properties. These included not only anchorage of keratin filament bundles but also nucleation of keratin filaments, which was also observed in migrating cells. The findings highlight the fundamental role of hemidesmosomal adhesion for keratin network formation and organization independent of other cytoskeletal filaments pointing to a unique mechanobiological function.
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