Evidence map›Paper›PMID 33669773›Full record

ArticleBiomolecules2021

Looking for Hidden Enemies of Metabarcoding: Species Composition, Habitat and Management Can Strongly Influence DNA Extraction while Examining Grassland Communities.

Anna Rucińska, Marcin Olszak, Sebastian Świerszcz, Marcin Nobis, Szymon Zubek, Grzegorz Kusza, Maja Boczkowska, Arkadiusz Nowak

Abstract read
In one paragraph

Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anna RucińskaPolish Academy of Sciences Botanical Garden, Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-976 Warszawa, Poland.
Marcin OlszakPolish Academy of Sciences Botanical Garden, Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-976 Warszawa, Poland.ORCID 0000-0003-3375-4506
Sebastian ŚwierszczPolish Academy of Sciences Botanical Garden, Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-976 Warszawa, Poland.ORCID 0000-0003-2035-0035
Marcin NobisInstitute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland.
Szymon ZubekInstitute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 3, 30-387 Kraków, Poland.ORCID 0000-0003-1338-9572
Grzegorz KuszaInstitute of Biology, University of Opole, Oleska 22, 45-052 Opole, Poland.
Maja BoczkowskaPolish Academy of Sciences Botanical Garden, Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-976 Warszawa, Poland.ORCID 0000-0001-8691-410X
Arkadiusz NowakPolish Academy of Sciences Botanical Garden, Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-976 Warszawa, Poland.

Funding

Narodowe Centrum Nauki 2017/25/B/NZ8/00572Narodowe Centrum Nauki 2019/35/B/NZ8/03358
6 · The paper itself

Abstract

Despite the raising preoccupation, the critical question of how the plant community is composed belowground still remains unresolved, particularly for the conservation priority types of vegetation. The usefulness of metabarcoding analysis of the belowground parts of the plant community is subjected to a considerable bias, that often impedes detection of all species in a sample due to insufficient DNA quality or quantity. In the presented study we have attempted to find environmental factors that determine the amount and quality of DNA extracted from total plant tissue from above- and belowground samples (1000 and 10,000 cm

Indexed as

DNA Barcoding, TaxonomicBiodiversityBiomassDNADNA, PlantEcologyEcosystemGrasslandHydrogen-Ion ConcentrationMagnesium OxideNitrogenPlant RootsPlantsPoaceaePolandRegression AnalysisDNADNA, PlantMagnesium OxideNitrogenSoilbelowground diversityCentral EuropeDNA extractionDNA qualitygraminoid vegetationroots

Identifiers

PMID33669773
PMCPMC7921978

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.