ArticlePathogens (Basel, Switzerland)2021
Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene.
Article in Pathogens (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 8 citations in OpenAlex.
- Toscana virus infection acquired in Campania, Italy, in a Hungarian traveller: case report with mini-review.Tropical diseases, travel medicine and vaccines · 2026Article
- Nine autochthonous cases of Toscana virus infection in France, 2022-2024: a clinical and virological overview.Journal of travel medicine · 2025Article
- Development and evaluation of a duplex RT-qPCR assay for the detection and identification of Mayaro and chikungunya viruses.Journal of clinical microbiology · 2025Article
- External quality assessment for molecular detection of sand fly-borne phleboviruses circulating in the Mediterranean Basin.Parasites & vectors · 2025Article
- Development of a novel amplicon based whole-genome sequencing framework for improved surveillance of Toscana virus.Communications biology · 2025Article
- Toscana virus - an emerging Mediterranean arbovirus transmitted by sand flies.The Journal of general virology · 2024Review
- Urine: A Pitfall for Molecular Detection of Toscana Virus? An Analytical Proof-of-Concept Study.Viruses · 2024Article
- Update in Diagnostics of Toscana Virus Infection in a Hyperendemic Region (Southern Spain).Viruses · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene. Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes. In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples. In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
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Registered trials
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