ArticlePloS one2021
Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Bioartificial human corneas generated by tissue engineering. A historical and technical review.Histology and histopathology · 2026Review
- Generation of a Bioengineered Substitute of the Human Sclero-Corneal Limbus Using a Novel Decellularization Method.Pharmaceutics · 2025Article
- Development of stromal corneal grafts using a novel decellularization method with sodium cocoyl glutamate onAnnals of translational medicine · 2025Article
- Decellularization of camel corneas for tissue engineering applications.International ophthalmology · 2025Article
- Advancing Bilateral Limbal Deficiency Surgery: A Comprehensive Review of Innovations with Mucosal Cells.Biomedicines · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction.Materials today. Bio · 2024Article
- Decellularized Matrices for the Treatment of Tissue Defects: from Matrix Origin to Immunological Mechanisms.Biomolecules & therapeutics · 2024Review
- Pig Models in Retinal Research and Retinal Disease.Cold Spring Harbor perspectives in medicine · 2024Review
- Replace or Regenerate? Diverse Approaches to Biomaterials for Treating Corneal Lesions.Biomimetics (Basel, Switzerland) · 2024Review
- Epithelial Immune Cell Response to Initial Soft Contact Lens Wear in the Human Corneal and Conjunctival Epithelium.Investigative ophthalmology & visual science · 2023Article
- A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste.Frontiers in bioengineering and biotechnology · 2023Article
- A proposed model of xeno-keratoplasty using 3D printing and decellularization.Frontiers in pharmacology · 2023Article
- Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization.Frontiers in bioengineering and biotechnology · 2023Article
- Induction of Corneal Epithelial Differentiation of Induced Pluripotent and Orbital Fat-Derived Stem Cells Seeded on Decellularized Human Corneas.Stem cell reviews and reports · 2022Article
- Decellularization of xenografted tumors provides cell-specificFrontiers in oncology · 2022Article
- Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds.Pharmaceutics · 2021Article
- A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.International journal of molecular sciences · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe aim of this study is to compare the three previously applied, conventional porcine corneal decellularization methods and to demonstrate the importance of preserving the corneal limbus through decellularization.
methodsFresh, wild-type (with or without) limbus porcine corneas were decellularized using three different methods, including (i) sodium dodecyl sulfate (SDS), (ii) hypertonic saline (HS), and (iii) N2 gas (NG). Post-treatment evaluation was carried out using histological, residual nuclear material, and ultrastructural analyses. Glycerol was used to help reduce the adverse effects of decellularization. The corneas were preserved for two weeks in cornea storage medium.
resultsAll three decellularization methods reduced the number of keratocytes at different rates in the stromal tissue. However, all methods, except SDS, resulted in the retention of large numbers of cells and cell fragments. The SDS method (0.1% SDS, 48h) resulted in almost 100% decellularization in corneas without limbus. Low decellularization capacity of the NG method (<50%) could make it unfavorable. Although HS method had a more balanced damage-decellularization ratio, its decellularization capacity was lower than SDS method. Preservation of the corneoscleral limbus could partially prevent structural damage and edema, but it would reduce the decellularization capacity.
conclusionOur results suggest that SDS is a very powerful decellularization method, but it damages the cornea irreversibly. Preserving the corneoscleral limbus reduces the efficiency of decellularization, but also reduces the damage.
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