ArticleArchives of toxicology2021
Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay.
Article in Archives of toxicology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Modulatory Effects of Cirsimarin on Lung Cancer A549 Cells Migration in 2D and 3D Cultures Involves Transcriptional Regulation of Metalloproteinases.Journal of biochemical and molecular toxicology · 2026Article
- Impact of cryopreservation on DNA damage in Acer platanoides L. seeds evaluated by the comet assay.Scientific reports · 2025Article
- Suppressed DNA repair capacity in flight attendants after air travel.Scientific reports · 2025Article
- Impact of -20 C° cryopreservation on serum factors from schistosomiasis patients at different storage durations: insights into serum bio-banking.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Comparison of DNA damage in fresh and frozen blood samples: implications for the comet assay in human biomonitoring studies.Archives of toxicology · 2024Article
- Evolution of Minimally Invasive and Non-Invasive Preimplantation Genetic Testing: An Overview.Journal of clinical medicine · 2024Review
- Air Pollution and Primary DNA Damage among Zagreb (Croatia) Residents: A Cross-Sectional Study.Journal of xenobiotics · 2024Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Cell survival after DNA damage in the comet assay.Archives of toxicology · 2021Article
- Differential DNA Damage Response of Peripheral Blood Lymphocyte Populations.Frontiers in immunology · 2021Article
- Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response inOxidative medicine and cellular longevity · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be useful to determine individual susceptibility in blood samples. However, in human biomonitoring studies, working with large sample numbers that are acquired over an extended time period requires some additional considerations. One of the most important issues is the storage of samples and its effect on the outcome of the comet assay. Another important question is the suitability of different blood preparations. In this study, we analysed the effect of cryopreservation on DNA damage and repair activity in human blood samples. In addition, we investigated the suitability of different blood preparations. The alkaline and FPG as well as two different types of repair comet assay and an in vitro hydrogen peroxide challenge were applied. Our results confirmed that cryopreserved blood preparations are suitable for investigating DNA damage in the alkaline and FPG comet assay in whole blood, buffy coat and PBMCs. Ex vivo hydrogen peroxide challenge yielded its optimal effect in isolated PBMCs. The utilised repair comet assay with either UVC or hydrogen peroxide-induced lesions and an aphidicolin block worked well in fresh PBMCs. Cryopreserved PBMCs could not be used immediately after thawing. However, a 16-h recovery with or without mitotic stimulation enabled the application of the repair comet assay, albeit only in a surviving cell fraction.
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