Evidence map›Paper›PMID 33666691›Full record

ArticleInternational journal of legal medicine2021

Evaluation of one-step RT-PCR multiplex assay for body fluid identification.

Qinrui Yang, Baonian Liu, Yuxiang Zhou, Yining Yao, Zhihan Zhou, Hui Li, Chengchen Shao, Kuan Sun, Hongmei Xu, Qiqun Tang and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in International journal of legal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Qinrui Yang *Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Baonian Liu *Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Yuxiang ZhouDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Yining YaoDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Zhihan ZhouDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Hui LiShanghai Key Laboratory of Crime Scene Evidence, Institute of Criminal Science and Technology, Shanghai Municipal Public Security Bureau, Shanghai, 200083, China.
Chengchen ShaoDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Kuan SunDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Hongmei XuDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Qiqun TangDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Yiwen ShenDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. shenyiwen@fudan.edu.cn.
Jianhui XieDepartment of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. jhxie@fudan.edu.cn.
Shanghai Medical College of Fudan University · CNFudan University · CNShanghai Public Security Bureau · CNShanghai University of Traditional Chinese Medicine · CN

Funding

National Natural Science Foundation of China 81571853Opening Project of Shanghai Key Laboratory of Crime Scene Evidence 2017XCWZK09Shanghai Sailing Program 18YF1421400
6 · The paper itself

Abstract

The discrimination of body fluid stains provides crucial evidence during the investigation of criminal cases. Previous studies have demonstrated the practical value of mRNA profiling in body fluid identification. Conventional strategy of mRNA profiling entails reverse transcription and PCR amplification in two separate procedures with different buffer systems. In this study, we subjected the one-step multiplex reverse transcription PCR strategy to mRNA profiling with the inclusion of the same 18 tissue-specific biomarkers in the F18plex system targeting peripheral blood, menstrual blood, vaginal secretion, saliva, semen, and urine. The Qiagen OneStep RT-PCR kit and Titanium One-Step RT-PCR kit were applied to multiplex construction, while reproducible profiling results were obtained with both kits. Compared to the F18plex system, similar expression profiles of biomarkers were obtained in targeted tissues, while expected cross-reaction was observed in non-targeted body fluids. However, CYP2B7P1 and SPINK5 were detected in menstrual blood samples, which was not observed using the F18plex system. Full-profiling results were obtained in all samples using 0.1 ng peripheral blood and semen RNA, and 1 ng menstrual blood, vaginal secretion, saliva, and urine RNA. In conclusion, the application of one-step mRNA profiling strategy could be a reliable and economical method for the simplified, specific, and simultaneous analysis of tissue-specific biomarkers for the discrimination of body fluid origin.

Indexed as

Gene Expression ProfilingBiomarkersBody FluidsFemaleHumansMaleMultiplex Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerBiomarkersRNA, MessengerBody fluid identificationmRNA profilingMultiplex assayOne-step RT-PCR

Identifiers

PMID33666691
OpenAlexW3133879323

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.