Evidence map›Paper›PMID 33665787›Full record

ArticleMolecular biology reports2021

The use of the cellular thermal shift assay for the detection of intracellular beta-site amyloid precursor protein cleaving enzyme-1 ligand binding.

Mark Chambers, Alexandré Delport, Raymond Hewer

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Discovery of novel KRAS‒PDEActa pharmaceutica Sinica. B · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Mark ChambersDiscipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, 3201, South Africa.ORCID http://orcid.org/0000-0002-3712-2752
Alexandré DelportDiscipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, 3201, South Africa.ORCID http://orcid.org/0000-0003-3511-4369
Raymond HewerDiscipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, 3201, South Africa. hewerr@ukzn.ac.za.ORCID http://orcid.org/0000-0002-6025-8826
University of KwaZulu-Natal · ZA

Funding

South African National Research Foundation grant UID: 116297South African National Research Foundation/Deutscher Akademischer Austauschdienst grant UID: 111619
6 · The paper itself

Abstract

Inhibition of the Alzheimer's disease associated protein β-site amyloid precursor protein cleaving enzyme-1 (BACE1) remains a potential avenue for treatment of this disease. The cellular thermal shift assay (CETSA) is an attractive method of screening for protein binding molecules due to its ability to detect intracellular binding while avoiding the need to purify the protein in question. Here, the CETSA was carried out using the known BACE1 inhibitor verubecestat, where an increase in T

Indexed as

Amyloid beta-Protein PrecursorAmyloid Precursor Protein SecretasesBiological AssayDimethyl SulfoxideHEK293 CellsHumansIntracellular SpaceLigandsAmyloid beta-Protein PrecursorAmyloid Precursor Protein SecretasesDimethyl SulfoxideLigandsAlzheimer’s diseaseBACE1CETSADSF

Identifiers

PMID33665787
OpenAlexW3133742403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.