Evidence map›Paper›PMID 33665631›Full record

ArticleSTAR protocols2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

Ioannis Skordos, Annelies Demeyer, Rudi Beyaert

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 30 citations in OpenAlex.

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  16. Ex Vivo Irradiation Protocol in Murine Lymph Node Cells.Bulletin of experimental biology and medicine · 2025
    Article
  17. A donor PD-1Signal transduction and targeted therapy · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ioannis SkordosCenter for Inflammation Research, VIB, Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium.
Annelies DemeyerCenter for Inflammation Research, VIB, Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium.
Rudi BeyaertCenter for Inflammation Research, VIB, Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium.
Ghent University · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

T cells play a key role in adaptive immunity. Defects in specific T cell receptors or signaling proteins can alter their frequency and activation status, which may be associated with immune disease or cancer. Monitoring of T cell frequency and function in genetically modified mice or murine models of disease is therefore of high interest. Here, we provide a detailed protocol to analyze regulatory T cells, T cell activation, and cytokine production in thymus, spleen, or blood via flow cytometry. For complete details on the use and execution of this protocol, please refer to Demeyer et al. (2020).

Indexed as

Flow CytometryLymphocyte ActivationAnimalsMiceMice, TransgenicSpleenT-Lymphocytes, RegulatoryFlow cytometry/mass cytometryImmunology

Identifiers

PMID33665631
PMCPMC7907922
OpenAlexW3130984355

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.