ArticleFrontiers in microbiology2021
Comparison of Two 16S rRNA Primers (V3-V4 and V4-V5) for Studies of Arctic Microbial Communities.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 2 of them syntheses that pooled it.
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Who cites it
84 citing papers in PubMed, 2 syntheses or guidelines pooled it, 162 citations in OpenAlex.
- Salivary microbial meta-analysis reveals gender differences in oral microbiota, core microbiota, and molecular markers.Frontiers in cellular and infection microbiology · 2026Pooled it
- Changes in the gut microbiota of patients with sarcopenia based on 16S rRNA gene sequencing: a systematic review and meta-analysis.Frontiers in nutrition · 2024Pooled it
- Dysbiosis in the Gut Microbiome of Pembrolizumab-Treated Non-Small Lung Cancer Patients Compared to Healthy Controls Characterized Through Opportunistic Sampling.Thoracic cancer · 2025Trial
- Characterisation of microbial succession for the estimation of PMI and PMSI in terrestrial and aquatic environments.Forensic science, medicine, and pathology · 2026Article
- Vertical Stratification in Composition, Metabolic Potential and Co-Occurrence Networks of Archaeal Communities in Mangrove Sediments, Zhanjiang, China.Ecology and evolution · 2026Article
- Multidimensional Differences and Driving Mechanisms of Bacterial Communities in Urban and Rural Rivers Across China.Microorganisms · 2026Article
- A Scalable and Cost-Effective In-Line Barcoding Strategy for Standardized 16S rRNA Gene Amplicon Sequencing: Performance Evaluation and Bias Assessment.Molecular ecology resources · 2026Article
- Kombucha inoculated fermentation reshapes microbial ecology and flavour metabolism in Yunnan Arabica coffee.NPJ science of food · 2026Article
- Shared Microbial Blueprints Underlying Symbiotic Plasticity in Desert Plant Endophytes.Microorganisms · 2026Article
- Oral Administration ofAnimals : an open access journal from MDPI · 2026Article
- Use of Microbial Biofilms to Assess Groundwater Quality in Karstic Ecosystems.Environmental microbiology · 2026Article
- Microbiome-Driven Mechanisms in Breast Cancer: Emerging Evidence From Gut Microbial Signatures to Therapeutic Response.BioMed research international · 2026Review
- The association between tongue features and tongue coating microbiota and gastrointestinal cancer: a systematic review and meta-analysis.Journal of oral microbiology · 2026Review
- Benthic bacteria communities of coral reefs are shaped by sediment properties rather than coral trophic state.PloS one · 2026Article
- Augmentation of psychrophilic anaerobic digestion with psychrotolerantFrontiers in microbiology · 2026Article
- Multi-omics interrogation of herbivorous phenotype acquisition in hybrid culter under a 30% crude protein diet.BMC genomics · 2025Article
- Too hot for my bugs: mediterranean heatwave disrupts associated microbiomes in the sponge Petrosia ficiformis.Environmental microbiome · 2025Article
- Microbiomes of frozen blood plasma samples reveal potential pathogens in wild birds and rodents.One health (Amsterdam, Netherlands) · 2025Article
- Vertical Structure and Functional Diversity of Microbial Communities in the Ross Sea, Antarctica.Microbial ecology · 2025Article
- Unveiling Bacterial Diversity in Portuguese Red Wine Effluents Through a Metagenomic Approach.Microorganisms · 2025Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial communities of the Arctic Ocean are poorly characterized in comparison to other aquatic environments as to their horizontal, vertical, and temporal turnover. Yet, recent studies showed that the Arctic marine ecosystem harbors unique microbial community members that are adapted to harsh environmental conditions, such as near-freezing temperatures and extreme seasonality. The gene for the small ribosomal subunit (16S rRNA) is commonly used to study the taxonomic composition of microbial communities in their natural environment. Several primer sets for this marker gene have been extensively tested across various sample sets, but these typically originated from low-latitude environments. An explicit evaluation of primer-set performances in representing the microbial communities of the Arctic Ocean is currently lacking. To select a suitable primer set for studying microbiomes of various Arctic marine habitats (sea ice, surface water, marine snow, deep ocean basin, and deep-sea sediment), we have conducted a performance comparison between two widely used primer sets, targeting different hypervariable regions of the 16S rRNA gene (V3-V4 and V4-V5). We observed that both primer sets were highly similar in representing the total microbial community composition down to genus rank, which was also confirmed independently by subgroup-specific catalyzed reporter deposition-fluorescence
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