Evidence map›Paper›PMID 33664667›Full record

ArticleFrontiers in pharmacology2021

The Species Identification in Traditional Herbal Patent Medicine, Wuhu San, Based on Shotgun Metabarcoding.

Jinxin Liu, Weishan Mu, Mengmeng Shi, Qing Zhao, Weijun Kong, Hongbo Xie, Linchun Shi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in pharmacology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
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  13. Characterization of the complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jinxin LiuHebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, China.
Weishan MuHebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, China.
Mengmeng ShiHebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, China.
Qing ZhaoHebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, China.
Weijun KongInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Hongbo XieHebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical University, Chengde, China.
Linchun ShiInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional herbal patent medicine typically consists of multiple ingredients, making it challenging to supervise contamination by impurities and the improper use of raw materials. This study employed shotgun metabarcoding for the species identification of biological ingredients in traditional herbal patent medicine, Wuhu San. The five prescribed herbal materials found in Wuhu San were collected, and their reference sequences were obtained by traditional DNA barcoding using Sanger sequencing. Two lab-made and three commercial Wuhu San samples were collected, and a total of 37.14 Gb of shotgun sequencing data was obtained for these five samples using the Illumina sequencing platform. A total of 1,421,013 paired-end reads were enriched for the Internal Transcribed Spacer 2 (ITS2),

Indexed as

DNA barcodingshotgun metabarcodingspecies identificationtraditional herbal patent medicineWuhu San

Identifiers

PMID33664667
PMCPMC7921783

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.