Evidence map›Paper›PMID 33663509›Full record

ArticleCancer cell international2021

Long non-coding RNA LINC00470 in serum derived exosome: a critical regulator for proliferation and autophagy in glioma cells.

Wenjia Ma, Yu Zhou, Min Liu, Qilin Qin, Yan Cui

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Wenjia MaDepartment of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Road, Changsha, 410011, Hunan, People's Republic of China.
Yu ZhouDepartment of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Road, Changsha, 410011, Hunan, People's Republic of China.
Min LiuDepartment of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Road, Changsha, 410011, Hunan, People's Republic of China.
Qilin QinDepartment of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Road, Changsha, 410011, Hunan, People's Republic of China.
Yan CuiDepartment of Neurosurgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Road, Changsha, 410011, Hunan, People's Republic of China. 148202093@csu.edu.cn.ORCID http://orcid.org/0000-0002-7665-4970
Central South University · CN

Funding

Hunan Provincial Health Commission Project Fund 202104040152Hunan Provincial Natural Science Youth Foundation 2019JJ50873
6 · The paper itself

Abstract

backgroundTo explore the mechanism of LINC00470 in serum exosomes from glioma patients regulating the autophagy and proliferation of glioma cells.

methodsExosomes were extracted from glioma patients (GBM-exo). Expression of LINC00470 in exosomes was analyzed with the clinicopathological characteristics of glioma patients. Glioma mouse model was established. The effects of LINC00470, miR-580-3p and WEE1 on cell autophagy and proliferation, as well as the activation of PI3K/AKT/mTOR pathway were measured. Dual luciferase reporter assay and RNA immunoprecipitation (RIP) were conducted to validate the binding of LINC00470 and miR-580-3p and of miR-580-3p and WEE1.

resultsLINC00470 overexpressed in GBM-exo and associated with disease severity and postoperative survival time of glioma patients. GBM-exo deteriorated tumor progression in nude mice. Cells incubated with GBM-exo or transfected with pcDNA3.1-LINC00470/miR-580-3p inhibitor/pcDNA3.1-WEE1 had less autophagosome, downregulated LC3-II/LC3-I and Beclin1 expression levels and increased expression of p62 as well as strengthened proliferation ability. The PI3K/AKT/mTOR pathway was activated. LINC00470 competitively bound to miR-580-3p with WEE1.

conclusionLINC00470 in GBM-exo can bind to miR-580-3p in glioma cells to regulate WEE1 expression and activate the PI3K/AKT/mTOR pathway, thereby inhibiting autophagy and enhancing the proliferation of glioma cells.

Indexed as

AutophagyExosomeGliomaLINC00470miR-580-3pPI3K/AKT/mTORProliferationWEE1

Identifiers

PMID33663509
PMCPMC7931344
OpenAlexW3133957299

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.