ArticleBMC veterinary research2021
Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia.
Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Identification of the Potential chi-miR-148a-5p-PTGS2 Regulatory Axis Mediating TNF and IL-17 Signaling Pathways in Caprine Bronchial Epithelial Cells Challenged with Klebsiella Pneumoniae.Veterinary sciences · 2026Article
- Utility of recombinant Schistosoma bovis 22.6 kDa antigen for diagnosis of human urogenital schistosomiasis by ELISA: a cross-sectional study in Yewa North LGA, Ogun State, Nigeria.BMC infectious diseases · 2026Article
- Integrated 16S rRNA and Metagenomic Analysis of Pulmonary Microbiota in Sheep with Pneumonia.Veterinary sciences · 2026Article
- A newly developed rapid K39-based LeishmaCHECK Ab ELISA for high-throughput serological screening of canine leishmaniasis.Current research in parasitology & vector-borne diseases · 2026Article
- Development of an indirect enzyme-linked immunosorbent assay based on the nucleocapsid protein of bovine parainfluenza virus type 3.Frontiers in cellular and infection microbiology · 2026Article
- Characterization of aFrontiers in cellular and infection microbiology · 2026Article
- Development of an indirect ELISA based on a new specific lipoprotein LP53 for the detection of antibodies against Mycoplasma synoviae.BMC veterinary research · 2025Article
- Development and Characterization of a Recombinant galT-galU Protein for Broad-Spectrum Immunoprotection Against Porcine Contagious Pleuropneumonia.International journal of molecular sciences · 2025Article
- Recent Advances in Pretreatment Methods and Detection Techniques for Veterinary Drug Residues in Animal-Derived Foods.Metabolites · 2025Review
- Development of a New Indirect ELISA Test for the Detection of Anti-Feline Coronavirus Antibodies in Cats.Veterinary sciences · 2025Article
- A New Rapid Indirect ELISA Test for Serological Diagnosis of Feline Immunodeficiency.Veterinary sciences · 2025Article
- Evaluation of a newly developed rapid ELISA to detect anti-Ehrlichia canis antibodies in dogs.Parasite (Paris, France) · 2025Article
- Investigating the immunological activity of the Hsp70-P113 fusion protein for Mycoplasma ovipneumoniae detection: a groundbreaking study.BMC veterinary research · 2024Article
- Phylogenetic analysis ofOpen veterinary journal · 2024Article
- Revealing microbial diversity in buffalo milk with high somatic cell counts: implications for mastitis diagnosis and treatment.Veterinary research communications · 2024Article
- A Duplex PCR Assay for Rapid Detection ofFoodborne pathogens and disease · 2024Article
- Staining-Enhanced Peroxidase-Mimicking Gold Nanoparticles in Nano-ELISA for Highly Sensitive Detection ofACS omega · 2023Article
- Capture ELISA for KPC Detection in Gram-Negative Bacilli: Development and Standardisation.Microorganisms · 2023Article
- Development and application of an indirect ELISA and nested PCR for the epidemiological analysis ofFrontiers in microbiology · 2023Article
- Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus.Microbial cell factories · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundKlebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are little systematic methods for detection of goat Klebsiella pneumoniae in livestock production.
resultsIn this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99 × 10
conclusionsTaken together, we successfully established an indirect ELISA method for detecting antibodies against Klebsiella pneumoniae in goats, which can be applied in production.
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