Evidence map›Paper›PMID 33662762›Full record

ArticleDNA repair2021

Tracking Escherichia coli DNA polymerase V to the entire genome during the SOS response.

Mahmood Faraz, Roger Woodgate, Anders R Clausen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Mahmood FarazDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, 40530 Gothenburg, Sweden.
Roger WoodgateLaboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892-3371, USA.
Anders R ClausenDepartment of Medical Biochemistry and Cell Biology, University of Gothenburg, 40530 Gothenburg, Sweden. Electronic address: anders.clausen@gu.se.
University of Gothenburg · SENational Institutes of Health · US

Funding

DNA Replication, Repair, and Mutagenesis In Eukaryotic And Prokaryotic CellsZIAHD001500 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI WOODGATE, ROGER · 2009 to 2025
$37.5M
Intramural NIH HHS ZIA HD001500
6 · The paper itself

Abstract

Ribonucleotides are frequently incorporated into DNA and can be used as a marker of DNA replication enzymology. To investigate on a genome-wide scale, how E. coli pol V accesses undamaged chromosomal DNA during the SOS response, we mapped the location of ribonucleotides incorporated by steric gate variants of pol V across the entire E. coli genome. To do so, we used strains that are deficient in ribonucleotide excision repair (ΔrnhB), deficient in pol IV DNA polymerase, constitutively express all SOS-regulated genes [lexA(Def)] and constitutively "activated" RecA* (recA730). The strains also harbor two steric gate variants of E. coli pol V (Y11A, or F10L), or a homolog of pol V, (pol V

Indexed as

Genome, BacterialSOS Response, GeneticsDNA, BacterialDNA-Directed DNA PolymeraseEscherichia coliEscherichia coli ProteinsDNA, BacterialDNA-Directed DNA PolymeraseDNA polymerase V, E coliEscherichia coli ProteinsUmuC protein, E coliUmuD protein, E coliDNA polymerase VR391Ribonucleotide Excision RepairRibonucleotide incorporationSOS responseSteric gate mutant

Identifiers

PMID33662762
PMCPMC8286053
OpenAlexW3133076812

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.