ArticleMicrobial biotechnology2021
Production of 11α-hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Stereoselective 15α-hydroxylation of androsta-1,4-diene-3,17-dione byRSC advances · 2026Article
- Combinatorial biosynthesis for the engineering of novel fungal natural products.Communications chemistry · 2024Review
- Current Trends and Perspectives in Microbial Bioconversions of Steroids.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- Microbial Steroid Production Technologies: Current Trends and Prospects.Microorganisms · 2021Article
- Production of 11α-hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis.Microbial biotechnology · 2021Article
- Engineering the Steroid Hydroxylating System fromMicroorganisms · 2021Article
- Comparison of bacterial communities and antibiotic resistance genes in oxidation ditches and membrane bioreactors.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
11α-hydroxylated steroid synthons are one of the most important commercially pharmaceutical intermediates used for the production of contraceptive drugs and glucocorticoids. These compounds are currently produced by biotransformation using fungal strains in two sequential fermentation steps. In this work, we have developed by a rational design new recombinant bacteria able to produce 11α-hydroxylated synthons in a single fermentation step using cholesterol (CHO) or phytosterols (PHYTO) as feedstock. We have designed a synthetic operon expressing the 11α-hydroxylating enzymes from the fungus Rhizopus oryzae that was cloned into engineered mutant strains of Mycolicibacterium smegmatis that were previously created to produce 4-androstene-3,17-dione (AD), 1,4-androstadiene-3,17-dione (ADD) from sterols. The introduction of the fungal synthetic operon in these modified bacterial chassis has allowed producing for the first time 11αOH-AD and 11αOH-ADD with high yields directly from sterols in a single fermentation step. Remarkably, the enzymes of sterol catabolic pathway from M. smegmatis recognized the 11α-hydroxylated intermediates as alternative substrates and were able to efficiently funnel sterols to the desired hydroxylated end-products.
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