ArticleNucleus (Austin, Tex.)2021
HP1β carries an acidic linker domain and requires H3K9me3 for phase separation.
Article in Nucleus (Austin, Tex.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Repeat-rich RNA guides repetitive genomic elements into biomolecular condensates for heterochromatin organization and muscle integrity.Nucleic acids research · 2026Article
- Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and composition.Nucleic acids research · 2025Article
- Tau Liquid-Liquid Phase Separation in Alzheimer's Disease: Mechanisms, Pathogenesis, and Therapeutic Implications.Molecular neurobiology · 2025Review
- HP1β and H3K9me3 Regulate Olfactory Receptor Choice and Transcriptional Identity.bioRxiv : the preprint server for biology · 2025Article
- HP1 loses its chromatin clustering and phase separation function across evolution.Nature communications · 2025Article
- Liquid‒liquid phase separation: a potentially fundamental mechanism of sepsis.Cell death discovery · 2025Review
- The HP1 box of KAP1 organizes HP1α for silencing of endogenous retroviral elements in embryonic stem cells.Nature communications · 2025Article
- Liquid-liquid phase separation in diseases.MedComm · 2024Review
- Genome folding principles uncovered in condensin-depleted mitotic chromosomes.Nature genetics · 2024Article
- Mechanistic drivers of chromatin organization into compartments.Current opinion in genetics & development · 2024Review
- Mode of SUV420H2 heterochromatin localization through multiple HP1 binding motifs in the heterochromatic targeting module.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Interplay between charge distribution and DNA in shaping HP1 paralog phase separation and localization.eLife · 2024Article
- Phase Separation of Chromatin Structure-related Biomolecules: A Driving Force for Epigenetic Regulations.Current protein & peptide science · 2024Review
- Multimodal interactions drive chromatin phase separation and compaction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Phase-separated CCER1 coordinates the histone-to-protamine transition and male fertility.Nature communications · 2023Article
- Heterochromatin organization and phase separation.Nucleus (Austin, Tex.) · 2023Review
- Genome folding principles revealed in condensin-depleted mitotic chromosomes.bioRxiv : the preprint server for biology · 2023Article
- Assessing the Phase Separation Propensity of Proteins in Living Cells Through Optodroplet Formation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- MeCP2-induced heterochromatin organization is driven by oligomerization-based liquid-liquid phase separation and restricted by DNA methylation.Nucleus (Austin, Tex.) · 2022Article
- Probing protein ubiquitination in live cells.Nucleic acids research · 2022Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation (LLPS) mediated formation of membraneless organelles has been proposed to coordinate biological processes in space and time. Previously, the formation of phase-separated droplets was described as a unique property of HP1α. Here, we demonstrate that the positive net charge of the intrinsically disordered hinge region (IDR-H) of HP1 proteins is critical for phase separation and that the exchange of four acidic amino acids is sufficient to confer LLPS properties to HP1β. Surprisingly, the addition of mono-nucleosomes promoted H3K9me3-dependent LLPS of HP1β which could be specifically disrupted with methylated but not acetylated H3K9 peptides. HP1β mutants defective in H3K9me3 binding were less efficient in phase separation
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.