Evidence map›Paper›PMID 33659900›Full record

ArticleSTAR protocols2021

Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells.

Sandeep Gupta, Ken Yamauchi, Bennett G Novitch, Samantha J Butler

Open access · goldAbstract read

In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it

0cells of the map it votes in

6citing papers in PubMed

0.5field-weighted citation impact, top 39% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

bioRxiv : the preprint server for biology · 2025
Article
Human induced pluripotent stem cell-derived therapies for regeneration after central nervous system injury.Neural regeneration research · 2025
Article
Investigating the basis of lineage decisions and developmental trajectories in the dorsal spinal cord through pseudotime analyses.Development (Cambridge, England) · 2024
Article
Accelerated differentiation of human induced pluripotent stem cells into regionally specific dorsal and ventral spinal neural progenitor cells for application in spinal cord therapeutics.Frontiers in neuroscience · 2023
Article
In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion.Cell reports · 2022
Article
Cell Transplantation for Repair of the Spinal Cord and Prospects for Generating Region-Specific Exogenic Neuronal Cells.Cell transplantation
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sandeep GuptaDepartment of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA.

Ken YamauchiDepartment of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA.

Bennett G NovitchDepartment of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA.

Samantha J ButlerDepartment of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA.

University of California, Los Angeles · US

Funding

UCLA IDDRC: Translational CoreP50HD103557 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Peyman Golshani, SUMA JACOB · 2020 to 2026

$9.6M

Diverse roles of the Bone Morphogenetic Proteins in the developing spinal cordR01NS085097 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUTLER, SAMANTHA J · 2014 to 2018

$1.7M

Molecular Pathways Controlling Respiratory Motor Neuron Formation and FunctionR01NS085227 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI NOVITCH, BENNETT G · 2015 to 2019

$1.6M

Notch-mediated modulation of Sonic hedgehog signaling in neural fate specification and differentiationR21NS115012 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI NOVITCH, BENNETT G · 2020 to 2021

$424k

NICHD NIH HHS P50 HD103557NINDS NIH HHS R01 NS085097NINDS NIH HHS R01 NS085227NINDS NIH HHS R21 NS115012

6 · The paper itself

Abstract

We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with bone morphogenetic protein 4 (RA+BMP4) is used to induce proprioceptive dI1s and mechanosensory dI3s in hPSC cultures. These protocols provide an important step toward developing therapies for regaining sensation in spinal cord injury patients. For complete details on the use and execution of this protocol, please refer to Gupta et al. (2018).

Indexed as

Bone Morphogenetic Protein 4Cell DifferentiationEmbryonic Stem CellsFlow CytometryHigh-Throughput Nucleotide SequencingHuman Embryonic Stem CellsHumansInduced Pluripotent Stem CellsInterneuronsNeuronsPluripotent Stem CellsSpineTretinoinBMP4 protein, humanBone Morphogenetic Protein 4TretinoinCell differentiationNeuroscienceStem cells

Identifiers

PMID33659900

DOI10.1016/j.xpro.2021.100319

PMCPMC7890043

OpenAlexW3126340159

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.