Evidence map›Paper›PMID 33659532›Full record

ArticleBio-protocol2020

Self-organization Assay for Min Proteins of

Shunshi Kohyama, Kei Fujiwara, Natsuhiko Yoshinaga, Nobuhide Doi

Open access · hybridAbstract read
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Shunshi KohyamaDepartment of Biosciences and Informatics, Keio University, Yokohama, Japan.
Kei FujiwaraDepartment of Biosciences and Informatics, Keio University, Yokohama, Japan.
Natsuhiko YoshinagaMathematical Science Group, WPI Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai, Japan.
Nobuhide DoiDepartment of Biosciences and Informatics, Keio University, Yokohama, Japan.
Keio University · JPAdvanced Institute of Materials Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Min system determines the cell division plane of bacteria. As a cue of spatiotemporal regulation, the Min system uses wave propagation of MinD protein (Min wave). Therefore, the reconstitution of the Min wave in cell-sized closed space will lead to the creation of artificial cells capable of cell division. The Min waves emerge via coupling between the reactions among MinD, MinE, and ATP and the differences in diffusion rate on the cell membrane and in the cytoplasm. Because Min waves appear only under the balanced condition of the reaction-diffusion coupling, special attentions are needed towards several technical points for the reconstitution of Min waves in artificial cells. This protocol describes a technical method for stably generating Min waves in artificial cells.

Indexed as

Artificial cellsCell divisionCell-sized spaceIn vitro reconstitutionMin systemReaction-diffusion couplingSpatiotemporal regulation

Identifiers

PMID33659532
PMCPMC7842281
OpenAlexW3010978871

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.