ArticleBio-protocol2020
Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria.
Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
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- In Vitro Preclinical Evaluation of BPP-PNA Antisense Antibiotics: A Protocol for Efficacy, Safety, and Resistance Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- LTX-315 is a novel broad-spectrum antimicrobial peptide against clinical multidrug-resistant bacteria.Journal of advanced research · 2025Article
- Cell Membrane-Targeted Antibacterial Synergy of Citric Acid-Sodium Hypochlorite AgainstFoods (Basel, Switzerland) · 2025Article
- South Tyrol (Italy)Molecules (Basel, Switzerland) · 2025Article
- Dual Redox Targeting by Pyrroloformamide A and Silver Ions Enhances Antibacterial and Anti-Biofilm Activity Against Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- The carRS-ompV-virK operon of Vibrio cholerae senses antimicrobial peptides and activates the expression of multiple resistance systems.Scientific reports · 2025Article
- Modulation of the mechanism of action of antibacterial silver N-heterocyclic carbene complexes by variation of the halide ligand.RSC advances · 2025Article
- Antimicrobial effect of benzbromarone combined with colistin against multidrug-resistant bacteria.Frontiers in medicine · 2025Article
- Transcriptomic investigations of polymyxins and colistin/sulbactam combination against carbapenem-resistantComputational and structural biotechnology journal · 2024Article
- IMT-P8 potentiates Gram-positive specific antibiotics in intrinsically resistant Gram-negative bacteria.Antimicrobial agents and chemotherapy · 2024Article
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Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to assess the change in bacterial membrane permeability after treatment. This study describes the protocol for the assays of outer and inner membrane permeability using the fluorescent probes N-phenyl-1-naphthylamine and propidium iodide. Compared with other experiments, such as electron microscopy and the assay of minimal bactericidal concentration, this methodology provides a simpler, faster, and cost-effective way of estimating the membrane-permeabilizing effect and bactericidal efficacy of antibacterial molecules. This study presents an optimized protocol with respect to the classical protocols by incubating bacteria with antibacterial molecules in the culture condition identical to that of antibacterial assays and then detecting the signal of the fluorescent probe in the buffer without broth and antibacterial molecules. This protocol avoids the effect of nutrient deficiency on the physiological status of bacteria and the interference of antibacterial molecules towards the fluorescent probe. Thus, this method can effectively and precisely evaluate the membrane permeability and match the results obtained from other antibacterial assays, such as minimum inhibitory concentration and time-kill curve assays.
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Registered trials
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