Evidence map›Paper›PMID 33659495›Full record

ArticleBio-protocol2020

Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from

Ryan R Cupo, James Shorter

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
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  6. RNA G-Quadruplexes Function as a Tunable Switch of FUS Phase Separation.bioRxiv : the preprint server for biology · 2025
    Article
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  11. Design principles to tailor Hsp104 therapeutics.bioRxiv : the preprint server for biology · 2024
    Article
  12. Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Ryan R CupoDepartment of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
James ShorterDepartment of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
University of Pennsylvania · US

Funding

STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008275 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI WAND, A. JOSHUA · 1988 to 2018
$6.0M
Defining the mechanistic basis of a prion disaggregaseR01GM099836 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI SHORTER, JAMES · 2013 to 2024
$3.9M
Isolating small-molecule enhancers of the human alpha-synuclein disaggregase machineryR21AG061784 · NIA · UNIVERSITY OF PENNSYLVANIA · PI SHORTER, JAMES · 2019 to 2020
$441k
Engineering mitochondrial protein disaggregases for neurodegenerative diseaseF31AG060672 · NIA · UNIVERSITY OF PENNSYLVANIA · PI CUPO, RYAN R · 2018 to 2020
$122k
NIA NIH HHS F31 AG060672NIA NIH HHS R21 AG061784NIGMS NIH HHS R01 GM099836NIGMS NIH HHS T32 GM008275
6 · The paper itself

Abstract

Skd3 (encoded by human

Indexed as

ChaperoneCLPBProtein aggregationProtein disaggregaseSkd3TEV

Identifiers

PMID33659495
PMCPMC7842574
OpenAlexW3107235366

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.