Evidence map›Paper›PMID 33659409›Full record

ArticleBio-protocol2020

Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue.

Ryoji Amamoto, Mauricio D Garcia, Emma R West, Jiho Choi, Sylvain W Lapan, Elizabeth A Lane, Norbert Perrimon, Constance L Cepko

Abstract read
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ryoji AmamotoDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Mauricio D GarciaDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Emma R WestDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Jiho ChoiDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Sylvain W LapanDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Elizabeth A LaneDepartment of Genetics, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Norbert PerrimonDepartment of Genetics, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.
Constance L CepkoDepartments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Blavatnik Institute, Harvard Medical School, Boston MA 02115, USA.

Funding

Regulation of Cell Type Identity in Retinal Bipolar Cell PrecursorsK99EY028215 · NEI · HARVARD MEDICAL SCHOOL · PI LAPAN, SYLVAIN · 2017 to 2018
$194k
NEI NIH HHS K99 EY028215
6 · The paper itself

Abstract

Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method for bulk transcriptional profiling of cell types for which cell type-specific genetic markers or antibodies are not readily available. We therefore developed Probe-Seq, which uses hybridization of gene-specific probes to RNA markers for isolation of specific types of cells, to enable downstream FACS isolation and bulk RNA sequencing. We show that this method can enable isolation and profiling of specific cell types from mouse retina, frozen human retina,

Indexed as

Cell TypesFISHRNARNA-sequencingTranscriptional Profiling

Identifiers

PMID33659409
PMCPMC7842722

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.