ArticleBio-protocol2020
Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in
Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Rapid analysis of strigolactone receptor activity in aPlant direct · 2022Article
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are separated during translation by a 2A "self-cleaving" peptide. This approach enables changes in the relative abundance of a target protein to be detected sensitively, reducing variability in expression of the ratiometric reporter transgene that may occur across different tissues or transformation events. We recently developed a set of Gateway-compatible plant transformation vectors termed pRATIO that combine a variety of promoters, fluorescent and bioluminescent reporters, and 2A peptides derived from foot-and-mouth disease virus. Here, we describe in detail how to use the dual-fluorescent ratiometric reporter pRATIO3212 to examine the relative abundance of a target protein after transient expression in
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