ArticleBio-protocol2020
Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution.
Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Reversible control of post-Golgi transport by brefeldin A reveals recycling endosome maturation during glycosylphosphatidylinositol-anchored protein transport.Nature communications · 2026Article
- Localization of Proteins in the Secretory Pathway of Pichia pastoris Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Golgi Retention and Oncogenic Signaling of KIT Tyrosine Kinase in Gastrointestinal Stromal Tumor.Sub-cellular biochemistry · 2026Review
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- Association of Golgi Stacks and Recycling Endosomes Ensures Post-Golgi Trafficking.Sub-cellular biochemistry · 2026Review
- Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants.Nature plants · 2025Article
- Molecular tag for promotingiScience · 2024Article
- Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging.Cell structure and function · 2024Article
- Dynamic movement of the Golgi unit and its glycosylation enzyme zones.Nature communications · 2024Article
- Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast.eLife · 2024Article
- Transport mechanisms between the endocytic, recycling, and biosynthetic pathways via endosomes and theFrontiers in cell and developmental biology · 2024Review
- Extremely high spatiotemporal resolution microscopy for live cell imaging by single photon counting, noise elimination, and a novel restoration algorithm based on probability calculation.Frontiers in cell and developmental biology · 2024Article
- The yeast endocytic early/sorting compartment exists as an independent sub-compartment within theeLife · 2023Article
- Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles.International journal of nanomedicine · 2022Review
- Super resolution live imaging: The key for unveiling the true dynamics of membrane traffic around the Golgi apparatus in plant cells.Frontiers in plant science · 2022Review
- The Golgi Apparatus and its Next-Door Neighbors.Frontiers in cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo proteins between organelles. For directly visualizing the events in membrane trafficking system, researchers have long awaited the technology that enables simultaneous multi-color and four-dimensional observation at high space and time resolution. Super-resolution microscopy methods, for example STED, PALM/STORM, and SIM, provide greater spatial resolution, however, these methods are not enough in temporal resolution. The super-resolution confocal live imaging microscopy (SCLIM) that we developed has now achieved the performance required. By using SCLIM, we have conducted high spatiotemporal visualization of secretory cargo together with early and late Golgi resident proteins tagged with three different fluorescence proteins. We have demonstrated that secretory cargo is indeed delivered within the Golgi by cisternal maturation. In addition, we have visualized details of secretory cargo trafficking in the Golgi, including formation of zones within a maturing cisterna, in which Golgi resident proteins are segregated, and movement of cargo between these zones. This protocol can be used for simultaneous three-color and four-dimensional observation of various phenomena in living cells, from yeast to higher plants and animals, at high spatiotemporal resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.