Evidence map›Paper›PMID 33659351›Full record

ArticleBio-protocol2020

RNA Stability Measurements Using RT-qPCR in

Tianran Jia, Brandon H Le

Abstract read
In one paragraph

Article in Bio-protocol, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Receptor kinase pathway signal tuning through a nontranscriptional incoherent feedforward loop.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Tianran JiaDepartment of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, United States of America.
Brandon H LeDepartment of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, California, United States of America.

Funding

Regulation of APETALA2 in flower development in ArabidopsisR01GM061146 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI CHEN, MENG · 2000 to 2022
$6.5M
NIGMS NIH HHS R01 GM061146
6 · The paper itself

Abstract

Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive method of mRNA stability measurement through quantitative reverse transcription PCR (RT-qPCR) after inhibition of RNA transcription by cordycepin in

Indexed as

ArabidopsisCordycepinPrimary MicroRNA TranscriptsRNA DegradationRNA StabilityRT-qPCR

Identifiers

PMID33659351
PMCPMC7842415

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.