ArticleBiomedical optics express2021
Hyperspectral microscopy combined with DAPI staining for the identification of hepatic carcinoma cells.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
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- AI-Powered Spectral Imaging for Virtual Pathology Staining.Bioengineering (Basel, Switzerland) · 2025Article
- A Novel Laboratory-Based Strategy for Single Adipocyte and Adipose-Derived Stem Cells Extraction for Transplantation: An Experimental Research.Aesthetic plastic surgery · 2025Article
- Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint.Journal of orthopaedic surgery and research · 2024Article
- SpeCamX: mobile app that turns unmodified smartphones into multispectral imagers.Biomedical optics express · 2023Article
- Compressive hyperspectral microscopy for cancer detection.Journal of biomedical optics · 2023Article
- Article
- Staging of Skin Cancer Based on Hyperspectral Microscopic Imaging and Machine Learning.Biosensors · 2022Article
- Identification of DAPI-stained normal, inflammatory, and carcinoma hepatic cells based on hyperspectral microscopy.Biomedical optics express · 2022Article
- Cancer detection from stained biopsies using high-speed spectral imaging.Biomedical optics express · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, the DAPI staining is firstly reported for use in the identification of hepatic carcinoma cells based on hyperspectral microscopy. Nuclei in cancer cells usually contain more aneuploidies than that in normal cells, leading to the change of DNA content. Here, we stain hepatic carcinoma tissues and normal hepatic tissues with 4',6-diamidino-2-phenylindole (DAPI) which is sensitive to the DNA content as a fluorochrome binds to DNA. Consequently, the difference in DNA content between hepatic carcinoma cells and normal hepatic cells can be identified by the fluorescent spectral characteristics. Harnessing the hyperspectral microscopy, we find that the fluorescent properties of these two kinds of cells are different not only in the intensity but also in the spectral shape. These properties are exploited to train a support vector machine (SVM) model for classifying cells. The results show that the sensitivity and specificity for the identification of 1000 hepatic carcinoma samples are 99.3% and 99.1%, respectively.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.