ArticleJournal of aerosol science2021
Optimization of an air-liquid interface
Article in Journal of aerosol science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Variation on Toxic and Inflammatory Potential of Coal Smoke of Different Sources From a Region With High Lung Cancer Risk.Environmental toxicology · 2026Article
- Toxic in vitro effects of flavoured-air inhalers containing essential oils in differentiated human nasal epithelial cells at air-liquid interface.Archives of toxicology · 2026Article
- Butyrate attenuates house dust mite-induced activation of 16HBE bronchial epithelial cells in association with modulation of gene expression.Respiratory research · 2026Article
- Alignment of in vitro and in vivo pulmonary inflammation models using crystalline quartz silica.Particle and fibre toxicology · 2026Article
- Micro- and Nanoplastics and Pulmonary Health: The Current State of Research.Microplastics (Basel, Switzerland) · 2026Article
- Combination of 3D Micro Contact Printing and Scraping Technique for Site Selective Cocultivation Across a Porous Micro Structured Scaffold.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- NAMs for closing knowledge gaps in assessment of respiratory uptake - a PARC project.Frontiers in toxicology · 2026Article
- A human relevant in vitro alveolar epithelial barrier model to assess inhaled pollutant hazard.Scientific reports · 2025Article
- Application of in vitro pulmonary models for hazard screening of silica particles.Archives of toxicology · 2025Article
- Biodegradable Nanofiber Membranes for Air-Liquid Interface Culture: Advancing AirwayACS omega · 2025Article
- Models to evaluate the pulmonary toxicity of desert dust and what we have learned from them so far: a mini-review.Naunyn-Schmiedeberg's archives of pharmacology · 2025Review
- Towards prospective identification of respiratory sensitizers: effects of piperazine, chloramine-T, and toluene-diisocyanate in an air-liquid interface model comprising human bronchial epithelial cells.Archives of toxicology · 2025Article
- Analysis of Polymer/siRNA Nanoparticle Efficacy and Biocompatibility in 3D Air-Liquid Interface Culture Compared to 2D Cell Culture.Pharmaceutics · 2025Article
- Two- and Three-Dimensional Culture Systems: Respiratory In Vitro Tissue Models for Chemical Screening and Risk-Based Decision Making.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Synergistic effects ofFrontiers in cellular and infection microbiology · 2025Article
- Precision-cut lung slices in air-liquid interface (PCLS-ALI): A novelbioRxiv : the preprint server for biology · 2024Article
- Carbon Nanotube Immunotoxicity in Alveolar Epithelial Type II Cells Is Mediated by Physical Contact-Independent Cell-Cell Interaction with Macrophages as Demonstrated in an Optimized Air-Liquid Interface (ALI) Coculture Model.Nanomaterials (Basel, Switzerland) · 2024Article
- Aerobic glycolysis of bronchial epithelial cells rewiresInfection and immunity · 2024Article
- Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend.PloS one · 2024Article
- Cell line-basedFrontiers in medicine · 2024Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhalation exposure to environmental and occupational aerosol contaminants is associated with many respiratory health problems. To realistically mimic long-term inhalation exposure for toxicity testing, lung epithelial cells need to maintained and exposed under air-liquid interface (ALI) conditions for a prolonged period of time. In addition, to study cellular responses to aerosol particles, lung epithelial cells have to be co-cultured with macrophages. To that aim, we evaluated human bronchial epithelial Calu-3, 16HBE14o- (16HBE), H292, and BEAS-2B cell lines with respect to epithelial morphology, barrier function and cell viability under prolonged ALI culture conditions. Only the Calu-3 cells can retain the monolayer structure and maintain a strong tight junction under long-term ALI culture at least up to 2 weeks. As such, Calu-3 cells were applied as the structural barrier to create co-culture models with human monocyte-derived macrophages (MDMs) and THP-1 derived macrophages (TDMs). Adhesion of macrophages onto the epithelial monolayer was allowed for 4 h with a density of 5 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.