ArticleProceedings of the National Academy of Sciences of the United States of America2021
Cas9-expressing chickens and pigs as resources for genome editing in livestock.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- Models of the human heart for biomedical research: Opportunities and challenges.Physiological reports · 2026Review
- Advancing immunity and disease resistance in chickens through genome editing.Journal of animal science and biotechnology · 2026Review
- Production of immune receptor knockout chickens via directAnimal biotechnology · 2025Article
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Development of CRISRP/Cas9-based TP53-knockout pig muscle stem cells for use in the cultured meat industry.3 Biotech · 2025Article
- Cas9-expressing cattle using the PiggyBac transposon all-in-one system.BMC genomics · 2025Article
- The avian embryo as a time-honoured animal model in developmental, biomedical and agricultural research.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Human ACE2 transgenic pigs are susceptible to SARS-CoV-2 and develop COVID-19-like disease.Nature communications · 2025Article
- Article
- Avian Models for Human Carcinogenesis-Recent Findings from Molecular and Clinical Research.Cells · 2024Review
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- Face processing in animal models: implications for autism spectrum disorder.Frontiers in neuroscience · 2024Review
- Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells.Frontiers in genome editing · 2024Article
- Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos.Molecular therapy. Nucleic acids · 2023Article
- Review
- Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation.Genome biology · 2023Article
- Modern Approaches to Mouse Genome Editing Using the CRISPR-Cas Toolbox and Their Applications in Functional Genomics and Translational Research.Advances in experimental medicine and biology · 2023Review
- Genome Editing in Pigs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- How genome editing changed the world of large animal research.Frontiers in genome editing · 2023Review
- Application of CRISPR for In Vivo Mouse Cancer Studies.Cancers · 2022Review
Corrections and comments
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Authors and funding
27 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.