ArticleMicrobial cell factories2021
Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 43 citations in OpenAlex.
- A workflow to explore elongase diversity and extend the repertoire of fatty acids produced by Yarrowia lipolytica.Microbial cell factories · 2025Article
- Application of vibrational spectroscopies as process analytical techniques for monitoring fermentation and the conversion of lignocellulosic biomass by oleaginous filamentous fungi.Microbial cell factories · 2025Article
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
- Calcium starvation leads to strain-specific gene regulation of lipid and carotenoid production in Mucor circinelloides.G3 (Bethesda, Md.) · 2025Article
- Enhanced co-production of extracellular biopolymers and intracellular lipids by Rhodotorula using lignocellulose hydrolysate and fish oil by-product urea.Biotechnology for biofuels and bioproducts · 2025Article
- Simultaneous production of fatty acids and amino polysaccharides from Norway spruce hydrolysates using oleaginous Mucor circinelloides.Scientific reports · 2025Article
- Lipid Production in Cultivable Filamentous Fungi Isolated from Antarctic Soils: A Comprehensive Study.Microorganisms · 2025Article
- Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies.Archives of microbiology · 2024Review
- Explorative characterization and taxonomy-aligned comparison of alterations in lipids and other biomolecules in Antarctic bacteria grown at different temperatures.Environmental microbiology reports · 2024Article
- Raman spectroscopy online monitoring of biomass production, intracellular metabolites and carbon substrates during submerged fermentation of oleaginous and carotenogenic microorganisms.Microbial cell factories · 2023Article
- Wood Degradation byJournal of fungi (Basel, Switzerland) · 2023Article
- Multiscale spectroscopic analysis of lipids in dimorphic and oleaginous Mucor circinelloides accommodate sustainable targeted lipid production.Fungal biology and biotechnology · 2023Article
- Article
- Deep learning-enabled Inference of 3D molecular absorption distribution of biological cells from IR spectra.Communications chemistry · 2022Article
- Lipid Readjustment inBiomolecules · 2022Article
- The Use of Constituent Spectra and Weighting in Extended Multiplicative Signal Correction in Infrared Spectroscopy.Molecules (Basel, Switzerland) · 2022Article
- Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi.Frontiers in nutrition · 2022Review
- Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Article
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6 authors at 1 institution in 1 country.
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Abstract
backgroundOleaginous filamentous fungi can accumulate large amount of cellular lipids and potentially serve as a major source of oleochemicals for food, feed, chemical, pharmaceutical, and transport industries. Transesterification of microbial oils is an essential step in microbial lipid production at both laboratory and industrial scale. Direct transesterification can considerably reduce costs, increase sample throughput and improve lipid yields (in particular fatty acid methyl esters, FAMEs). There is a need for the assessment of the direct transesterification methods on a biomass of filamentous fungi due to their unique properties, specifically resilient cell wall and wide range of lipid content and composition. In this study we have evaluated and optimised three common direct transesterification methods and assessed their suitability for processing of fungal biomass.
resultsThe methods, based on hydrochloric acid (Lewis method), sulphuric acid (Wahlen method), and acetyl chloride (Lepage method), were evaluated on six different strains of Mucoromycota fungi by using different internal standards for gas chromatography measurements. Moreover, Fourier transform infrared (FTIR) spectroscopy was used for the detection of residual lipids in the biomass after the transesterification reaction/extraction, while transesterification efficiency was evaluated by nuclear magnetic resonance spectroscopy. The results show that the majority of lipids, in particular triglycerides, were extracted for all methods, though several methods had substandard transesterification yields. Lewis method, optimised with respect to solvent to co-solvent ratio and reaction time, as well as Lepage method, offer precise estimate of FAME-based lipids in fungal biomass.
conclusionsThe results show that Lepage and Lewis methods are suitable for lipid analysis of oleaginous filamentous fungi. The significant difference in lipid yields results, obtained by optimised and standard Lewis methods, indicates that some of the previously reported lipid yields for oleaginous filamentous fungi must be corrected upwards. The study demonstrates value of biomass monitoring by FTIR, importance of optimal solvent to co-solvent ratio, as well as careful selection and implementation of internal standards for gas chromatography.
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