Revealing the link between macrophage in microenvironment of osteosarcoma and poor prognosis by utilizing the Integrated analysis.

Sikuan Zheng, Jie Peng, Jingyu Jia, Tianlong Wu, Xigao Cheng

Open access · greenAbstract readMeta-AnalysisSystematic Review

In one paragraph

Synthesis in Journal of musculoskeletal & neuronal interactions, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

0.2field-weighted citation impact, top 51% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Sikuan ZhengDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, China.

Jie PengJiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, China.

Jingyu JiaDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, China.

Tianlong WuDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, China.

Xigao ChengDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, China.

Nanchang University · CNSecond Affiliated Hospital of Nanchang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesOsteosarcoma (OS) is the most common type of primary malignant bone tumor, The effect of tumor microenvironment components on OS oncogenesis remains unknown.

methodsTo investigate the function of immune cells in osteosarcoma, we provided a text-based GMT (Gene Matrix Transposed) file in which each line defines one of lm22 with their markers. We used STRING to draw DEG's PPI network and selected hub genes and modules. Then, survival analysis was conducted to hub genes. We identified 10,390 common genes, and identified 218 DEGs based on the combined t-value and Z scores.

resultsThe KEGG and GSEA enrichment analysis showed that macrophages are significantly activated in osteosarcoma. PPI network analysis revealed that hub gene CD163 molecule. We found that the expression of CD163 was negatively associated with the OS of osteosarcoma patients. These results suggest that macrophages are a risk factor in patients with osteosarcoma.

conclusionsThis study has systematically validated results of the studies carried out previously and filled up the gap in the field of OS on large-scaled meta-analysis. In addition, for the hub gene (CD163) and the macrophage cell capable of being used as a novel biomarker in promoting early diagnosis and development of therapeutic approaches.

Indexed as

Databases, GeneticAntigens, CDAntigens, Differentiation, MyelomonocyticBone NeoplasmsCD163 AntigenGene Regulatory NetworksHumansImmunity, CellularMacrophagesOsteosarcomaPrognosisProtein Array AnalysisReceptors, Cell SurfaceTumor MicroenvironmentAntigens, CDAntigens, Differentiation, MyelomonocyticCD163 AntigenReceptors, Cell SurfaceCD163 MoleculeMacrophage ActivationMacrophagesOsteosarcoma

Identifiers

PMID33657763

PMCPMC8020028

OpenAlexW3133711268

What OpenQuestion holds

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LicenceCC BY-NC-SA

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.