ArticleThe journal of physical chemistry. B2021
Effect of Buffer on Protein Stability in Aqueous Solutions: A Simple Protein Aggregation Model.
Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.
- BioMThermDB 1.0: Thermophysical Database of Proteins in Solutions.International journal of molecular sciences · 2022Pooled it
- Improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis method for quantifying the 11S/7S ratio in soybean storage proteins.Journal of the science of food and agriculture · 2026Article
- Comprehensive Biophysical Profiling Evidences Self-Oligomerization of Bacterially Expressed Pc Protein.Chembiochem : a European journal of chemical biology · 2026Article
- Modeling Opposite Effects of an Additive on Liquid-Liquid Phase Separation and Crystal Solubility of Protein Solutions.Molecules (Basel, Switzerland) · 2026Article
- Determinants of extraction efficiency in human amniotic membrane processing: biological, mechanical, and biochemical factors shaping growth factor yield.Cell and tissue banking · 2026Review
- IscS Kinetics in Native Mass Spectrometry Buffers Reveal Key Physiochemical Properties that Influence Enzyme Activity.Journal of the American Society for Mass Spectrometry · 2026Article
- The reliability of malaria rapid diagnostic test kit for detecting P. falciparum PfHRP2 in dried blood spot samples preserved under different conditions and durations.BMC research notes · 2025Article
- Influence of α-Helical Content on the Thermodiffusion of Apomyoglobin.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Identification of an uncharacterized protein as a novel regulator ofJournal of virology · 2025Article
- Optimizing experimental conditions: the role of buffered environments in microbial isolation, physiological studies, and taxonomic characterization.Applied and environmental microbiology · 2025Review
- Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A Review on the Stability Challenges of Advanced Biologic Therapeutics.Pharmaceutics · 2025Review
- Revisiting the Effect of Trypsin Digestion Buffers on Artificial Deamidation.Journal of the American Society for Mass Spectrometry · 2025Article
- Optimization of Black Garlic Protein Extraction Process and Exploration of Its Properties and Functions with Enzymatic Hydrolysis Products.Molecules (Basel, Switzerland) · 2024Article
- The influence of glycine onZeitschrift fur physikalische Chemie (Frankfurt am Main, Germany) · 2024Article
- Understanding the Phase Behavior of a Multistimuli-Responsive Elastin-like Polymer: Insights from Dynamic Light Scattering Analysis.The journal of physical chemistry. B · 2024Article
- Investigating the influence of solvent type and pH on protein adsorption onto silica surface by evanescent-wave cavity ring-down spectroscopy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Generation of Rapid and High-Quality Serum by Recombinant Prothrombin Activator Ecarin (RAPClot™).Biomolecules · 2024Article
- Buffer choice and pH strongly influence phase separation of SARS-CoV-2 nucleocapsid with RNA.Molecular biology of the cell · 2024Article
- Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment.Chemical reviews · 2024Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Liquid-liquid phase separation (LLPS) of proteins has recently been associated with the onset of numerous diseases. Despite several studies in this area of protein aggregation, buffer-specific effects always seem to be overlooked. In this study we investigated the influence of buffers on the phase stability of hen egg-white lysozyme (HEWL) and its respective protein-protein interactions by measuring the cloud point temperature, second virial coefficient, and interaction diffusion coefficient of several HEWL-buffer solutions (MOPS, phosphate, HEPES, cacodylate) at pH 7.0. The results indicate that the buffer molecules, depending on their hydration, adsorb on the protein surface, and modulate their electrostatic stability. The obtained information was used to extend the recently developed coarse-grained protein model to incorporate buffer-specific effects. Treated by Wertheim's perturbation theory the model qualitatively correctly predicted the experimentally observed phase separation of all investigated HEWL-buffer solutions, and further allowed us to predict the phase stability of protein formulations even in experimentally unattainable conditions. Since the theory can be straightforwardly extended to include multiple components it presents a useful tool to study protein aggregation in crowded cell-like systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.