Evidence map›Paper›PMID 33656437›Full record

ArticleMicrobial genomics2021

Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing

Bert Bogaerts, Stéphanie Nouws, Bavo Verhaegen, Sarah Denayer, Julien Van Braekel, Raf Winand, Qiang Fu, Florence Crombé, Denis Piérard, Kathleen Marchal and 3 more

Abstract readValidation Study
In one paragraph

Article in Microbial genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Retrospective surveillance of viableFrontiers in microbiology · 2023
    Article
  20. Transforming Shiga toxin-producingFrontiers in microbiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Bert BogaertsTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Stéphanie NouwsTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Bavo VerhaegenNational Reference Laboratory for Shiga toxin-producing Escherichia coli (NRL STEC), Foodborne Pathogens, Sciensano, Brussels, Belgium.
Sarah DenayerNational Reference Laboratory for Shiga toxin-producing Escherichia coli (NRL STEC), Foodborne Pathogens, Sciensano, Brussels, Belgium.
Julien Van BraekelTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Raf WinandTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Qiang FuTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Florence CrombéNational Reference Center for Shiga toxin-producing Escherichia coli (NRC STEC), Brussels, Belgium.
Denis PiérardNational Reference Center for Shiga toxin-producing Escherichia coli (NRC STEC), Brussels, Belgium.
Kathleen MarchalDepartment of Information Technology, IDLab, Ghent University, IMEC, Ghent, Belgium.
Nancy H C RoosensTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Sigrid C J De KeersmaeckerTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.
Kevin VannesteTransversal activities in Applied Genomics, Sciensano, Brussels, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Whole genome sequencing (WGS) enables complete characterization of bacterial pathogenic isolates at single nucleotide resolution, making it the ultimate tool for routine surveillance and outbreak investigation. The lack of standardization, and the variation regarding bioinformatics workflows and parameters, however, complicates interoperability among (inter)national laboratories. We present a validation strategy applied to a bioinformatics workflow for Illumina data that performs complete characterization of Shiga toxin-producing

Indexed as

Computational BiologyDNA, BacterialEscherichia coli InfectionsGenome, BacterialHumansSequence Analysis, DNAShiga-Toxigenic Escherichia coliWhole Genome SequencingWorkflowDNA, BacterialEscherichia colipublic healthSTEC, foodborne pathogensvalidationwhole genome sequencing

Identifiers

PMID33656437
PMCPMC8190621

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.