Evidence map›Paper›PMID 33655600›Full record

ArticleNeurogastroenterology and motility2021

The α isoform of cGMP-dependent protein kinase 1 (PKG1α) is expressed and functionally important in intrinsic primary afferent neurons of the guinea pig enteric nervous system.

Zhi S Li, Lin Y Hung, Kara G Margolis, Richard T Ambron, Ying J Sung, Michael D Gershon

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurogastroenterology and motility, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Three-dimensional-engineered bioprintedBioactive materials · 2022
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Zhi S LiDepartments of Pathology & Cell Biology, Columbia University, New York, NY, USA.
Lin Y HungDepartments of Pediatrics, Columbia University, New York, NY, USA.
Kara G MargolisDepartments of Pediatrics, Columbia University, New York, NY, USA.
Richard T AmbronDepartments of Pathology & Cell Biology, Columbia University, New York, NY, USA.
Ying J SungDepartments of Basic Science, The Commonwealth Medical College, Scranton, PA, USA.
Michael D GershonDepartments of Pathology & Cell Biology, Columbia University, New York, NY, USA.ORCID 0000-0002-5893-7643
Columbia University Irving Medical Center · USColumbia University · USGeisinger Commonwealth School of Medicine · US

Funding

MICROENVIRONMENT IN ENTERIC NEURON DEVELOPMENTR01NS015547 · NINDS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GERSHON, MICHAEL D, MARGOLIS, KARA GROSS · 1985 to 2022
$7.2M
NEURAL CONTROL OF GASTROINTESTINAL ACTIVITYR01NS012969 · NINDS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GERSHON, MICHAEL D · 1985 to 2012
$5.3M
Linkage of s100a10 (p11) to enteric 5-HT4-mediated serotonergic signaling roles in GI motility, enteric nervous system development, and co-morbid dysfunction of gut and brainR01DK126644 · NIDDK · NEW YORK UNIVERSITY · PI MARGOLIS, KARA GROSS · 2021 to 2024
$1.9M
Enteric Neuronal Development as a Determinant of Intestinal InflammationK08DK093786 · NIDDK · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MARGOLIS, KARA GROSS · 2013 to 2017
$781k
NIDDK NIH HHS K08 DK093786NIDDK NIH HHS R01 DK126644NINDS NIH HHS R01 NS012969NINDS NIH HHS R01 NS015547
6 · The paper itself

Abstract

backgroundIntrinsic primary afferent neurons (IPANs) enable the gut to manifest reflexes in the absence of CNS input. PKG1α is selectively expressed in a subset of neurons in dorsal root ganglia (DRG) and has been linked to nociception and long-term hyperexcitability.

methodsWe used immunoblotting, immunocytochemistry, and in vitro assays of IPAN-dependent enteric functions to test hypotheses that subsets of primary neurons of the ENS and DRG share a reliance on PKG1α expression. KEY

resultsPKG1α immunoreactivity was demonstrated in immunoblots from isolated myenteric ganglia. PKG1α, but not PKG1β, immunoreactivity, was coincident with that of neuronal markers (HuC/D; β3-tubulin) in both enteric plexuses. PKG1α immunoreactivity also co-localized with the immunoreactivities of the IPAN markers, calbindin (100%; myenteric plexus) and cytoplasmic NeuN (98 ± 1% submucosal plexus). CGRP-immunoreactive DRG neurons, identified as visceral afferents by retrograde transport, were PKG1α-immunoreactive. We used intraluminal cholera toxin to determine whether PKG1α was necessary to enable stimulation of the mucosa to activate Fos in enteric neurons. Tetrodotoxin (1.0 µM), low Ca CONCLUSIONS & INFERENCES: These data suggest that PKG1α is present and functionally important in IPANs and visceral afferent nociceptive neurons.

Indexed as

AnimalsCyclic GMP-Dependent Protein Kinase Type IEnteric Nervous SystemFemaleGastrointestinal MotilityGuinea PigsIntestinesMaleMyenteric PlexusNeurons, AfferentProtein IsoformsProto-Oncogene Proteins c-fosCyclic GMP-Dependent Protein Kinase Type IProtein IsoformsProto-Oncogene Proteins c-fosDRGgNG15547 astrointestinal motilityinflammatory bowel diseaseirritable bowel syndromePKG1α inhibitor

Identifiers

PMID33655600
PMCPMC8681866
OpenAlexW3134087127

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.