Evidence map›Paper›PMID 33655280›Full record

ArticlemedRxiv : the preprint server for health sciences2021

SARS-CoV-2 genomic surveillance identifies naturally occurring truncations of ORF7a that limit immune suppression.

Artem Nemudryi, Anna Nemudraia, Tanner Wiegand, Joseph Nichols, Deann T Snyder, Jodi F Hedges, Calvin Cicha, Helen Lee, Karl K Vanderwood, Diane Bimczok and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Artem NemudryiDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Anna NemudraiaDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Tanner WiegandDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Joseph NicholsDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Deann T SnyderDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Jodi F HedgesDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Calvin CichaDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Helen LeeDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Karl K VanderwoodGallatin City-County Health Department, Bozeman, MT, 59715, USA.
Diane BimczokDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Mark JutilaDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
Blake WiedenheftDepartment of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.

Funding

Structural and functional understanding of bacterial defense and viral counter defenseR35GM134867 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Blake A Wiedenheft · 2020 to 2026
$3.8M
NIGMS NIH HHS R35 GM134867
6 · The paper itself

Abstract

Over 200,000 whole genome sequences of SARS-CoV-2 have been determined for viruses isolated from around the world. These sequences have been critical for understanding the spread and evolution of SARS-CoV-2. Using global phylogenomics, we show that mutations frequently occur in the C-terminal end of ORF7a. We have isolated one of these mutant viruses from a patient sample and used viral challenge experiments to demonstrate that Δ115 mutation results in a growth defect. ORF7a has been implicated in immune modulation, and we show that the C-terminal truncation results in distinct changes in interferon stimulated gene expression. Collectively, this work indicates that ORF7a mutations occur frequently and that these changes affect viral mechanisms responsible for suppressing the immune response.

Indexed as

IFN responseORF7aSARS-CoV-2

Identifiers

PMID33655280
PMCPMC7924305

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.