Mapping the Mechanome-A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression.

Vina D L Putra, Iman Jalilian, Madeline Campbell, Kate Poole, Renee Whan, Florence Tomasetig, Melissa L Knothe Tate

Open access · hybridAbstract read

In one paragraph

Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.1field-weighted citation impact, top 52% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment.Communications biology · 2023
Review
Mechanomics Approaches to Understand Cell Behavior in Context of Tissue Neogenesis, During Prenatal Development and Postnatal Healing.Frontiers in cell and developmental biology · 2019
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Vina D L PutraMechBio Team, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.

Iman JalilianMechBio Team, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.

Madeline CampbellMechBio Team, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.

Kate PooleCellular Mechanotransduction Group, EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia.

Renee WhanMark Wainwright Analytical Centre, University of New South Wales, Sydney, Australia.

Florence TomasetigMark Wainwright Analytical Centre, University of New South Wales, Sydney, Australia.

Melissa L Knothe TateMechBio Team, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia.

UNSW Sydney · AUEMBL Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mechanomics, the mechanics equivalent of genomics, is a burgeoning field studying mechanical modulation of stem cell behavior and lineage commitment. Analogous to mechanical testing of a living material as it adapts and evolves, mapping of the mechanome necessitates the development of new protocols to assess changes in structure and function in live stem cells as they adapt and differentiate. Previous techniques have relied on imaging of cellular structures in fixed cells and/or live cell imaging of single cells with separate studies of changes in mechanical and biological properties. Here we present two complementary protocols to study mechanobiology and mechanoadaptation of live stem cells in adherent and motile contexts. First, we developed and tested live imaging protocols for simultaneous visualization and tracking of actin and tubulin mechanoadaptation as well as shape and volume of cells and their nuclei in adherent model embryonic murine mesenchymal stem cells (C3H/10T1/2) and in a neuroblastoma cell line. Then we applied the protocol to enable quantitative study of primary human mesenchymal stem cells in a motile state,

Indexed as

ActinCell ingressionCytoskeletal dynamicsDynamic cytoskeletal adaptationLive cell imagingMechanoadaptationMechanomicsNucleus and cell shape and volumeTubulin

Identifiers

PMID33654934

DOI10.21769/BioProtoc.3439

PMCPMC7853984

OpenAlexW2994569236

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.