Evidence map›Paper›PMID 33654913›Full record

ArticleBio-protocol2019

Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.

Julia K Doh, Young Hwan Chang, Caroline A Enns, Claudia S Lόpez, Kimberly E Beatty

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Julia K DohDepartment of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon 97239, USA.
Young Hwan ChangDepartment of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon 97239, USA.
Caroline A EnnsDepartment of Cell, Developmental, and Cancer Biology, Oregon Health & Science University, Portland, Oregon 97239, USA.
Claudia S LόpezDepartment of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon 97239, USA.
Kimberly E BeattyDepartment of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon 97239, USA.
Oregon Health & Science University · US

Funding

Understanding the origins of rapid recurrence of pancreatic cancer after resectionP30CA069533 · NCI · OREGON HEALTH & SCIENCE UNIVERSITY · PI Grace B Gill · 1997 to 2026
$60.5M
New Technology for Tracking Proteins by Light and Electron MicroscopyR01GM122854 · NIGMS · OREGON HEALTH & SCIENCE UNIVERSITY · PI BEATTY, KIMBERLY ELIZABETH · 2017 to 2021
$2.1M
NCI NIH HHS P30 CA069533NIGMS NIH HHS R01 GM122854
6 · The paper itself

Abstract

Advances in fluorescence microscopy (FM), electron microscopy (EM), and correlative light and EM (CLEM) offer unprecedented opportunities for studying diverse proteins and nanostructures involved in fundamental cell biology. It is now possible to visualize and quantify the spatial organization of cellular proteins and other macromolecules by FM, EM, and CLEM. However, tagging and tracking cellular proteins across size scales is restricted by the scarcity of methods for attaching appropriate reporter chemistries to target proteins. Namely, there are few genetic tags compatible with EM. To overcome these issues we developed Versatile Interacting Peptide (VIP) tags, genetically-encoded peptide tags that can be used to image proteins by fluorescence and EM. VIPER, a VIP tag, can be used to label cellular proteins with bright, photo-stable fluorophores for FM or electron-dense nanoparticles for EM. In this Bio-Protocol, we provide an instructional guide for implementing VIPER for imaging a cell-surface receptor by CLEM. This protocol is complemented by two other Bio-Protocols outlining the use of VIPER ( Doh

Indexed as

Cell biologyCLEMElectron microscopyFluorescenceMicroscopyPeptideProtein tagQuantum dot

Identifiers

PMID33654913
PMCPMC7853974
OpenAlexW2988654873

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.